Protein Info for LRK53_RS00725 in Rhodanobacter sp000427505 FW510-R12

Annotation: DHA2 family efflux MFS transporter permease subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 508 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details transmembrane" amino acids 51 to 70 (20 residues), see Phobius details amino acids 79 to 101 (23 residues), see Phobius details amino acids 104 to 106 (3 residues), see Phobius details amino acids 108 to 129 (22 residues), see Phobius details amino acids 137 to 159 (23 residues), see Phobius details amino acids 165 to 187 (23 residues), see Phobius details amino acids 199 to 218 (20 residues), see Phobius details amino acids 230 to 249 (20 residues), see Phobius details amino acids 270 to 292 (23 residues), see Phobius details amino acids 303 to 322 (20 residues), see Phobius details amino acids 334 to 354 (21 residues), see Phobius details amino acids 366 to 385 (20 residues), see Phobius details amino acids 397 to 418 (22 residues), see Phobius details amino acids 475 to 493 (19 residues), see Phobius details TIGR00711: drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family" amino acids 13 to 492 (480 residues), 501.9 bits, see alignment E=9.3e-155 PF07690: MFS_1" amino acids 17 to 410 (394 residues), 175.6 bits, see alignment E=7.2e-56

Best Hits

Swiss-Prot: 49% identical to FARB_NEIGO: Fatty acid resistance protein FarB (farB) from Neisseria gonorrhoeae

KEGG orthology group: K03446, MFS transporter, DHA2 family, multidrug resistance protein B (inferred from 71% identity to psu:Psesu_0987)

MetaCyc: 51% identical to multidrug efflux pump membrane subunit EmrB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-363; TRANS-RXN-364; TRANS-RXN-365

Predicted SEED Role

"Inner membrane component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (508 amino acids)

>LRK53_RS00725 DHA2 family efflux MFS transporter permease subunit (Rhodanobacter sp000427505 FW510-R12)
MTTEFRPPHLALSTVGLSLATFMQVLDTTIANVSLPTIAGNLGVSNNQSTWVITSFAVSM
AIALPLTGFLSRRFGEVRLFTACTLLFALTSFLCGVSQSMGMLILFRALQGAVAGPMYPI
TQSLLIGIYPPAKRGMALALLAMVTVVAPIAGPILGGWITDNYSWPWIFFINVPIGIFAS
MVVANQLRAKVEKTERPKVDYVGLVSLIIGVGALQIVLDKGNDEDWFNSSFIIVTSIVSA
IGIAVFLIWELTDKDPIVDLRLFRHRNFTAGTIALTLAYAAFFAIGLLVPLWLQRNLGYT
STWAGFATAPIGVLPVLLTFFVGKYATRFDLRMLASVAFVVMAGTCFMRSDFFIGIDFYH
VAMVQLWQGLGVALFFMPVLTILLSDLQQNEIASGSGLATFLRTLGGSFSASLTTLLWER
RAVTHHERLTEHITAYSPTTQAALHQLGQGDQQAAAGVLNDIITQQAYQISFNEVFHALG
WIFLALVLVIWLARPPFTPKARPVAGGH