Protein Info for LRK53_RS00140 in Rhodanobacter sp000427505 FW510-R12

Annotation: class 1 fructose-bisphosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00316: FBPase
  • amino acids 6 to 197 (192 residues), 165.1 bits, E=1.5e-52
• PF18913: FBPase_C
  • amino acids 202 to 334 (133 residues), 149.8 bits, E=3.6e-48

Best Hits

Swiss-Prot: 70% identical to F16PA_XANOM: Fructose-1,6-bisphosphatase class 1 (fbp) from Xanthomonas oryzae pv. oryzae (strain MAFF 311018)

KEGG orthology group: K03841, fructose-1,6-bisphosphatase I [EC: 3.1.3.11] (inferred from 70% identity to xoo:XOO0015)

MetaCyc: 42% identical to fructose-1,6-bisphosphatase (Arabidopsis thaliana col)
Fructose-bisphosphatase. [EC: 3.1.3.11]

Predicted SEED Role

"Fructose-1,6-bisphosphatase, type I (EC 3.1.3.11)" in subsystem Calvin-Benson cycle or Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes (EC 3.1.3.11)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (23/26 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (16/17 steps found)
• glycolysis II (from fructose 6-phosphate) (11/11 steps found)
• glycolysis I (from glucose 6-phosphate) (12/13 steps found)
• superpathway of anaerobic sucrose degradation (16/19 steps found)
• gluconeogenesis I (11/13 steps found)
• formaldehyde assimilation III (dihydroxyacetone cycle) (10/12 steps found)
• gluconeogenesis III (10/12 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (15/19 steps found)
• superpathway of N-acetylneuraminate degradation (17/22 steps found)
• Calvin-Benson-Bassham cycle (10/13 steps found)
• sucrose biosynthesis I (from photosynthesis) (7/9 steps found)
• superpathway of hexitol degradation (bacteria) (13/18 steps found)
• ethene biosynthesis V (engineered) (18/25 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (19/27 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (18/26 steps found)
• oxygenic photosynthesis (11/17 steps found)

KEGG Metabolic Maps

• Biosynthesis of phenylpropanoids
• Carbon fixation in photosynthetic organisms
• Fructose and mannose metabolism
• Glycolysis / Gluconeogenesis
• Pentose phosphate pathway

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.3.11

Protein Sequence (343 amino acids)

>LRK53_RS00140 class 1 fructose-bisphosphatase (Rhodanobacter sp000427505 FW510-R12)
MKPVSLIQFLIEERRAGHINAELSLLIEVVARACKRIGVATGKGALGGVLGMAGTADSIS
MNIQGEAQKKLDVISNEILLEANAWGGHLAACASEEMEDPQQIPDGYPKGKHLLLFDPLD
GSSNIDVNISVGTIFSVLRCPDGVTEPKAEHFLQPGTTQLAAGYVVYGPSTVLVLTFGFG
THEFTLDREVGSFILSRRDIRIPVETAEFAINMSNQRHWQAPMQRYIGELLAGKEGPRGK
DFNMRWVASMVADVHRIVTRGGVFYYPLDAKIEKQGGKLRIMYEANPMAFIIEQAGGAAT
TGRSRIMELQPGGLHQRVPVFLGSKAEVEVATHYHLEADGALG