Protein Info for KEDOAH_26495 in Escherichia coli ECRC99

Annotation: alpha/beta hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 309 PF02129: Peptidase_S15" amino acids 15 to 140 (126 residues), 41.8 bits, see alignment E=3.8e-14 PF01738: DLH" amino acids 28 to 139 (112 residues), 30.9 bits, see alignment E=7.3e-11 PF00561: Abhydrolase_1" amino acids 49 to 251 (203 residues), 30.9 bits, see alignment E=7.9e-11 PF12146: Hydrolase_4" amino acids 53 to 135 (83 residues), 29.4 bits, see alignment E=1.7e-10 PF00326: Peptidase_S9" amino acids 56 to 140 (85 residues), 24.9 bits, see alignment E=4.6e-09 PF05448: AXE1" amino acids 90 to 137 (48 residues), 24.4 bits, see alignment 3.8e-09 PF08840: BAAT_C" amino acids 95 to 143 (49 residues), 21.6 bits, see alignment 7.2e-08

Best Hits

KEGG orthology group: K06889, (no description) (inferred from 99% identity to eoh:ECO103_1493)

Predicted SEED Role

"Dienelactone hydrolase and related enzymes"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (309 amino acids)

>KEDOAH_26495 alpha/beta hydrolase (Escherichia coli ECRC99)
MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCKEQTSGNIYGKALAEKG
YVVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLPYVDAERIGVLGVCGGGGY
AINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALNAMASQRTKEARGGELQINEL
LPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFTEVLL
TQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAVQEALEK
VIPFFNTHL