Protein Info for KEDOAH_25325 in Escherichia coli ECRC99

Name: emtA
Annotation: membrane-bound lytic murein transglycosylase EmtA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 203 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details PF01464: SLT" amino acids 43 to 168 (126 residues), 98.5 bits, see alignment E=9.6e-33

Best Hits

Swiss-Prot: 100% identical to EMTA_ECO5E: Endo-type membrane-bound lytic murein transglycosylase A (emtA) from Escherichia coli O157:H7 (strain EC4115 / EHEC)

KEGG orthology group: K08308, membrane-bound lytic murein transglycosylase E [EC: 3.2.1.-] (inferred from 99% identity to eco:b1193)

MetaCyc: 99% identical to lytic murein transglycosylase E (Escherichia coli K-12 substr. MG1655)
4.2.2.f [EC: 4.2.2.f]; 4.2.2.f [EC: 4.2.2.f]

Predicted SEED Role

"Membrane-bound lytic murein transglycosylase E (EC 3.2.1.-)" (EC 3.2.1.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.-

Use Curated BLAST to search for 3.2.1.- or 4.2.2.f

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (203 amino acids)

>KEDOAH_25325 membrane-bound lytic murein transglycosylase EmtA (Escherichia coli ECRC99)
VKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQKAGAAWGVDPQLITAI
IAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEPTTSELKNPERNISMGAAY
LNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSDRKKAISKINDLDADEFLDHV
ARNHPAPQAPRYIYKLEQALDAM