Protein Info for KEDOAH_25150 in Escherichia coli ECRC99

Name: bluF
Annotation: blue light-responsive regulator BluF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 403 transmembrane" amino acids 298 to 317 (20 residues), see Phobius details PF04940: BLUF" amino acids 2 to 92 (91 residues), 84.9 bits, see alignment E=3.6e-28 PF00563: EAL" amino acids 173 to 388 (216 residues), 168.1 bits, see alignment E=2.2e-53

Best Hits

Swiss-Prot: 98% identical to BLUF_ECOLI: Blue light- and temperature-regulated antirepressor BluF (bluF) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 98% identity to eco:b1163)

Predicted SEED Role

"Hypothetical protein ycgF"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (403 amino acids)

>KEDOAH_25150 blue light-responsive regulator BluF (Escherichia coli ECRC99)
MLTTLIYRSHIRDDEPVKKTEEMVSIANRRNMQSDVTGILLFNGSHFFQLLEGPEEQVKM
IYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHERDDVLQAVFDKGTSKFQLT
YDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKELDSCTLSPTINDHFAFHPIV
DPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTADLKSKALAFAMAHALELGDKMIS
INLLPMTLVNEPDAVSFLLDEIKANGLVPEQIIVEFTESEVISRFDEFAEAVKSLKAAGI
SVAIDHFGAGFAGLLLLSRFQPDRIKISQELITNVHKSGARQAIIQAIIKCCTSLEIQVS
AMGVATPEEWMWLESAGIEMFQGDLFAKAKLNGIPSVAWPEKK