Protein Info for KEDOAH_24705 in Escherichia coli ECRC99

Name: potB
Annotation: spermidine/putrescine ABC transporter permease PotB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 287 transmembrane" amino acids 10 to 36 (27 residues), see Phobius details amino acids 66 to 90 (25 residues), see Phobius details amino acids 100 to 124 (25 residues), see Phobius details amino acids 144 to 170 (27 residues), see Phobius details amino acids 198 to 224 (27 residues), see Phobius details amino acids 252 to 272 (21 residues), see Phobius details PF00528: BPD_transp_1" amino acids 83 to 280 (198 residues), 52.7 bits, see alignment E=2.3e-18

Best Hits

Swiss-Prot: 96% identical to POTB_SALTI: Spermidine/putrescine transport system permease protein PotB (potB) from Salmonella typhi

KEGG orthology group: K11071, spermidine/putrescine transport system permease protein (inferred from 100% identity to ecl:EcolC_2478)

MetaCyc: 99% identical to spermidine preferential ABC transporter membrane subunit PotB (Escherichia coli K-12 substr. MG1655)
ABC-25-RXN [EC: 7.6.2.11, 7.6.2.16]; 7.6.2.11 [EC: 7.6.2.11, 7.6.2.16]

Predicted SEED Role

"Spermidine Putrescine ABC transporter permease component PotB (TC 3.A.1.11.1)" in subsystem Polyamine Metabolism (TC 3.A.1.11.1)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.6.2.11 or 7.6.2.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (287 amino acids)

>KEDOAH_24705 spermidine/putrescine ABC transporter permease PotB (Escherichia coli ECRC99)
MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLARDDASFVKMVFTLDNYTRLLDPL
YFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLLFLLIVPFWTNSLIRIYGL
KIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYILLPFMVMPLYSSIEKLDKPL
LEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPAMGLFYVSDLMGGAKNLLIGNVI
KVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASRLLNKKATEIDN