Protein Info for KEDOAH_24205 in Escherichia coli ECRC99

Name: tmk
Annotation: dTMP kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00041: dTMP kinase
  • amino acids 2 to 200 (199 residues), 233.2 bits, E=1.1e-73
• PF02223: Thymidylate_kin
  • amino acids 8 to 200 (193 residues), 203.9 bits, E=9.2e-65

Best Hits

Swiss-Prot: 100% identical to KTHY_ECO5E: Thymidylate kinase (tmk) from Escherichia coli O157:H7 (strain EC4115 / EHEC)

KEGG orthology group: K00943, dTMP kinase [EC: 2.7.4.9] (inferred from 99% identity to eco:b1098)

MetaCyc: 99% identical to Tmk (Escherichia coli K-12 substr. MG1655)
T(2)-induced deoxynucleotide kinase. [EC: 2.7.4.12, 2.7.4.13, 2.7.4.9]

Predicted SEED Role

"Thymidylate kinase (EC 2.7.4.9)" (EC 2.7.4.9)

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis II (7/7 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• superpathway of pyrimidine deoxyribonucleoside salvage (8/9 steps found)
• pyrimidine deoxyribonucleotide phosphorylation (4/4 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• purine deoxyribonucleosides salvage (4/6 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.4.12 or 2.7.4.13 or 2.7.4.9

Protein Sequence (213 amino acids)

>KEDOAH_24205 dTMP kinase (Escherichia coli ECRC99)
MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGTQLAEKLRSLVLDIKSV
GDEIITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHDLSTQAYQGGGRGIDQHML
ATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIEQESFDFFNRTRARYLELAAQ
DKSIHTIDATQPLEAVMDAIRTTVTRWVKELDA