Protein Info for KEDOAH_24120 in Escherichia coli ECRC99
Name: flgJ
Annotation: flagellar assembly peptidoglycan hydrolase FlgJ
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to FLGJ_ECO57: Peptidoglycan hydrolase FlgJ (flgJ) from Escherichia coli O157:H7
KEGG orthology group: K02395, flagellar protein FlgJ (inferred from 99% identity to eco:b1081)MetaCyc: 83% identical to flagellar rod assembly protein/beta-N-acetylglucosaminidase (Salmonella enterica enterica serovar Typhimurium str. LT2)
Beta-N-acetylhexosaminidase. [EC: 3.2.1.52]
Predicted SEED Role
"Flagellar protein FlgJ [peptidoglycan hydrolase] (EC 3.2.1.-)" in subsystem Flagellum (EC 3.2.1.-)
MetaCyc Pathways
- peptidoglycan recycling I (14/14 steps found)
- peptidoglycan recycling II (7/10 steps found)
- chitin degradation II (Vibrio) (2/6 steps found)
- chitin degradation III (Serratia) (2/7 steps found)
KEGG Metabolic Maps
- Aminosugars metabolism
- Ascorbate and aldarate metabolism
- Glycosaminoglycan degradation
- Glycosphingolipid biosynthesis - ganglio series
- Glycosphingolipid biosynthesis - globo series
- Nucleotide sugars metabolism
- Other glycan degradation
- Ubiquinone and menaquinone biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 3.2.1.-, 3.2.1.52
Use Curated BLAST to search for 3.2.1.- or 3.2.1.52
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (313 amino acids)
>KEDOAH_24120 flagellar assembly peptidoglycan hydrolase FlgJ (Escherichia coli ECRC99) MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMFVQMMLKSMRDALPKDG LFSSEHTRLYTSMYDQQIAQQMTTGKGLGLAEMMVKQMTPEQPLPEESTPAAPMKFPLET VVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPAQLASQQSGVPHHLILAQAAL ESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITTTEYENGEAKKVKAKFRVYSSYL EALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAGYATDPHYARKLTNMIQQMKSISDK VSKTYSMNIDNLF