Protein Info for KEDOAH_23445 in Escherichia coli ECRC99

Name: ureC
Annotation: urease subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 567 PF00449: Urease_alpha" amino acids 3 to 119 (117 residues), 185.5 bits, see alignment E=6e-59 TIGR01792: urease, alpha subunit" amino acids 4 to 567 (564 residues), 964.1 bits, see alignment E=1.3e-294 PF01979: Amidohydro_1" amino acids 125 to 453 (329 residues), 280.5 bits, see alignment E=3.3e-87 PF07969: Amidohydro_3" amino acids 390 to 448 (59 residues), 31 bits, see alignment 3e-11

Best Hits

Swiss-Prot: 100% identical to URE1_ECO57: Urease subunit alpha (ureC1) from Escherichia coli O157:H7

KEGG orthology group: K01428, urease subunit alpha [EC: 3.5.1.5] (inferred from 100% identity to etw:ECSP_1251)

MetaCyc: 70% identical to urease alpha subunit (Sinorhizobium meliloti Rm2011)
Urease. [EC: 3.5.1.5]

Predicted SEED Role

"Urease alpha subunit (EC 3.5.1.5)" in subsystem Urea decomposition (EC 3.5.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.5

Use Curated BLAST to search for 3.5.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (567 amino acids)

>KEDOAH_23445 urease subunit alpha (Escherichia coli ECRC99)
MSNISRQAYADMFGPTTGDKIRLADTELWIEVEDDLTTYGEEVKFGGGKVIRDGMGQGQM
LSAGCADLVLTNALIIDYWGIVKADIGVKDGRIFAIGKAGNPDIQPNVTIPIGVSTEIIA
AEGRIVTAGGVDTHIHWICPQQAEEALTSGITTMIGGGTGPTAGSNATTCTPGPWYIYQM
LQAADSLPVNIGLLGKGNCSNPDALREQVAAGVIGLKIHEDWGATPAVINCALTVADEMD
VQVALHSDTLNESGFVEDTLTAIGGRTIHTFHTEGAGGGHAPDIITACAHPNILPSSTNP
TLPYTVNTIDEHLDMLMVCHHLDPDIAEDVAFAESRIRQETIAAEDVLHDLGAFSLTSSD
SQAMGRVGEVVLRTWQVAHRMKVQRGPLPEESGDNDNVRVKRYIAKYTINPALTHGIAHE
VGSIEVGKLADLVLWSPAFFGVKPATIVKGGMIAMAPMGDINGSIPTPQPVHYRPMFAAL
GSARHRCRVTFLSQAAAANGVAEQLNLHSTTAVVKGCRTVQKADMRHNSLLPDITVDSQT
YEVRINGELITSEPADILPMAQRYFLF