Protein Info for KEDOAH_22940 in Escherichia coli ECRC99
Name: hyaB
Annotation: Ni/Fe-hydrogenase large subunit
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to MBHL_SHIFL: Hydrogenase-1 large chain (hyaB) from Shigella flexneri
KEGG orthology group: K06281, hydrogenase large subunit [EC: 1.12.99.6] (inferred from 100% identity to eco:b0973)MetaCyc: 100% identical to hydrogenase 1 large subunit (Escherichia coli K-12 substr. MG1655)
1.12.98.-; Hydrogen:quinone oxidoreductase. [EC: 1.12.5.1]
Predicted SEED Role
"Uptake hydrogenase large subunit (EC 1.12.99.6)" in subsystem Hydrogenases or Membrane-bound Ni, Fe-hydrogenase (EC 1.12.99.6)
MetaCyc Pathways
- hydrogen oxidation I (aerobic) (1/1 steps found)
- hydrogen to dimethyl sulfoxide electron transfer (1/2 steps found)
- hydrogen to fumarate electron transfer (1/2 steps found)
- hydrogen to trimethylamine N-oxide electron transfer (1/2 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.12.99.6
Use Curated BLAST to search for 1.12.5.1 or 1.12.99.6
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (597 amino acids)
>KEDOAH_22940 Ni/Fe-hydrogenase large subunit (Escherichia coli ECRC99) MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVITNAVSCGTMFRGLEIIL QGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDNANIIRNIMLATLWCHDHL VHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSPGYFFDVQNRLKKFVEGGQLG IFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVKIHAVFGGKNPHPNWIVGGMPCA INIDESGAVGAVNMERLNLVQSIITRTADFINNVMIPDALAIGQFNKPWSEIGTGLSDKC VLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNVLPVDLVDPQQVQEFVDHAWYRYPNDQ VGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQERYSWIKAPRWRGNAMEVGPLARTLIAY HKGDAATVESVDRMMSALNLPLSGIQSTLGRILCRAHEAQWAAGKLQYFFNKLMTNLKNG NLATASTEKWEPTTWPTECRGVGFTEAPRGALGHWAAIRDGKIDLYQCVVPTTWNASPRD PKGQIGAYEAALMNTKMAIPEQPLEILRTLHSFDPCLACSTHVLGDDGSELISVQVR