Protein Info for KEDOAH_19820 in Escherichia coli ECRC99

Name: adk
Annotation: adenylate kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01351: adenylate kinase
  • amino acids 22 to 233 (212 residues), 254.2 bits, E=4.6e-80
• PF00406: ADK
  • amino acids 25 to 206 (182 residues), 204.5 bits, E=1.4e-64
• PF13207: AAA_17
  • amino acids 26 to 144 (119 residues), 98.1 bits, E=8.5e-32
• PF05191: ADK_lid
  • amino acids 143 to 178 (36 residues), 62.1 bits, 5.5e-21

Best Hits

Swiss-Prot: 100% identical to KAD_SHIB3: Adenylate kinase (adk) from Shigella boydii serotype 18 (strain CDC 3083-94 / BS512)

KEGG orthology group: K00939, adenylate kinase [EC: 2.7.4.3] (inferred from 100% identity to eok:G2583_0586)

MetaCyc: 100% identical to adenylate kinase (Escherichia coli K-12 substr. MG1655)
Nucleoside-diphosphate kinase. [EC: 2.7.4.6]; 2.7.4.6 [EC: 2.7.4.6]; 2.7.4.6 [EC: 2.7.4.6]; 2.7.4.6 [EC: 2.7.4.6]; 2.7.4.6 [EC: 2.7.4.6]; 2.7.4.6 [EC: 2.7.4.6]; Nucleoside-phosphate kinase. [EC: 2.7.4.6, 2.7.4.4]; Adenylate kinase. [EC: 2.7.4.6, 2.7.4.4, 2.7.4.3]

Predicted SEED Role

"Adenylate kinase (EC 2.7.4.3)" in subsystem Purine conversions (EC 2.7.4.3)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (26/26 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• superpathway of purine nucleotide salvage (13/14 steps found)
• superpathway of pyrimidine ribonucleosides salvage (10/10 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis II (8/8 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis II (7/7 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis II (7/7 steps found)
• ppGpp metabolism (6/6 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis I (6/6 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• superpathway of pyrimidine deoxyribonucleoside salvage (8/9 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis I (5/5 steps found)
• adenosine deoxyribonucleotides de novo biosynthesis II (4/4 steps found)
• guanosine deoxyribonucleotides de novo biosynthesis II (4/4 steps found)
• guanosine ribonucleotides de novo biosynthesis (4/4 steps found)
• pyrimidine deoxyribonucleotide phosphorylation (4/4 steps found)
• superpathway of pyrimidine nucleobases salvage (4/4 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• UTP and CTP de novo biosynthesis (3/3 steps found)
• adenosine ribonucleotides de novo biosynthesis (3/3 steps found)
• CMP phosphorylation (2/2 steps found)
• adenosine deoxyribonucleotides de novo biosynthesis I (2/2 steps found)
• guanosine deoxyribonucleotides de novo biosynthesis I (2/2 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• purine deoxyribonucleosides salvage (4/6 steps found)
• dZTP biosynthesis (3/5 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.4.6

Use Curated BLAST to search for 2.7.4.3 or 2.7.4.4 or 2.7.4.6

Protein Sequence (234 amino acids)

>KEDOAH_19820 adenylate kinase (Escherichia coli ECRC99)
MVVSFIAFSKNSTHFKGIFAMRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVK
SGSELGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAG
INVDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG