Protein Info for KEDOAH_15715 in Escherichia coli ECRC99

Name: dsbD
Annotation: protein-disulfide reductase DsbD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 565 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details transmembrane" amino acids 167 to 194 (28 residues), see Phobius details amino acids 207 to 231 (25 residues), see Phobius details amino acids 243 to 267 (25 residues), see Phobius details amino acids 288 to 317 (30 residues), see Phobius details amino acids 329 to 351 (23 residues), see Phobius details amino acids 363 to 381 (19 residues), see Phobius details amino acids 387 to 406 (20 residues), see Phobius details amino acids 415 to 431 (17 residues), see Phobius details PF11412: DsbD_N" amino acids 28 to 138 (111 residues), 126.3 bits, see alignment E=2e-40 PF13386: DsbD_2" amino acids 170 to 358 (189 residues), 31.1 bits, see alignment E=6.7e-11 PF02683: DsbD" amino acids 171 to 376 (206 residues), 67.5 bits, see alignment E=4.9e-22 PF13899: Thioredoxin_7" amino acids 464 to 536 (73 residues), 48.9 bits, see alignment E=1.8e-16 PF13098: Thioredoxin_2" amino acids 465 to 560 (96 residues), 53.2 bits, see alignment E=9.8e-18

Best Hits

Swiss-Prot: 100% identical to DSBD_ECO57: Thiol:disulfide interchange protein DsbD (dsbD) from Escherichia coli O157:H7

KEGG orthology group: K04084, thiol:disulfide interchange protein DsbD [EC: 1.8.1.8] (inferred from 99% identity to eco:b4136)

MetaCyc: 99% identical to thiol-disulfide exchange protein DsbD (Escherichia coli K-12 substr. MG1655)
RXN-19949 [EC: 1.8.4.16]; 1.8.4.16 [EC: 1.8.4.16]; 1.8.4.16 [EC: 1.8.4.16]; RXN0-7311 [EC: 1.8.4.16]

Predicted SEED Role

"Cytochrome c-type biogenesis protein DsbD, protein-disulfide reductase (EC 1.8.1.8)" in subsystem Biogenesis of c-type cytochromes or Periplasmic disulfide interchange (EC 1.8.1.8)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.8

Use Curated BLAST to search for 1.8.1.8 or 1.8.4.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (565 amino acids)

>KEDOAH_15715 protein-disulfide reductase DsbD (Escherichia coli ECRC99)
MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPVDQAFAFDFQQNQHDLNLTWQIKDGYYL
YRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTLPVTINQASAGATLTVTYQ
GCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQPTAQLPFSALWALLIGIGIAFT
PCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMALTYTALGLVVAAAGLQFQAALQ
HPYVLIGLAIVFTLLAMSMFGLLTLQLPSSLQTRLTLMSNRQQGGSPGGVFIMGTIAGLI
CSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYALGMGLPLMLITVFGNRLLPKSGPWMEQ
VKTAFGFVILALPVFLLERVIGDVWGLRLWSALGVAFFGWAFITSLQAKRGWMRVVQIIL
LAAALVSVRPLQDWAFGATHTAQTQTHLNFTQIKTVDELNQALVEAKGKPVMLDLYADWC
VACKEFEKYTFSDPQVQKALADTVLLQANVTANDAQDVALLKHLNVLGLPTILFFDGQGQ
EHPQARVTGFMDAETFSAHLRDRQP