Protein Info for KEDOAH_13625 in Escherichia coli ECRC99
Name: rffM
Annotation: lipopolysaccharide N-acetylmannosaminouronosyltransferase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to WECG_ECO57: UDP-N-acetyl-D-mannosaminuronic acid transferase (wecG) from Escherichia coli O157:H7
KEGG orthology group: K02852, UDP-N-acetyl-D-mannosaminuronic acid transferase [EC: 2.4.1.-] (inferred from 99% identity to ebr:ECB_03673)MetaCyc: 99% identical to UDP-N-acetyl-D-mannosaminuronic acid transferase (Escherichia coli K-12 substr. MG1655)
Lipopolysaccharide N-acetylmannosaminouronosyltransferase. [EC: 2.4.1.180]
Predicted SEED Role
"Probable UDP-N-acetyl-D-mannosaminuronic acid transferase (EC 2.4.1.-)" (EC 2.4.1.-)
MetaCyc Pathways
- superpathway of enterobacterial common antigen biosynthesis (9/10 steps found)
- enterobacterial common antigen biosynthesis (4/5 steps found)
KEGG Metabolic Maps
- Anthocyanin biosynthesis
- Carotenoid biosynthesis - General
- Flavone and flavonol biosynthesis
- Flavonoid biosynthesis
- Fructose and mannose metabolism
- Glycerolipid metabolism
- Glycosphingolipid biosynthesis - ganglio series
- Glycosphingolipid biosynthesis - globo series
- Glycosphingolipid biosynthesis - lacto and neolacto series
- Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
- High-mannose type N-glycan biosynthesis
- Keratan sulfate biosynthesis
- Lipopolysaccharide biosynthesis
- N-Glycan biosynthesis
- O-Glycan biosynthesis
- O-Mannosyl glycan biosynthesis
- Peptidoglycan biosynthesis
- Sphingolipid metabolism
- Zeatin biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 2.4.1.-
Use Curated BLAST to search for 2.4.1.- or 2.4.1.180
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (246 amino acids)
>KEDOAH_13625 lipopolysaccharide N-acetylmannosaminouronosyltransferase (Escherichia coli ECRC99) MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAINAEKMLTIEENAEVRE LINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMARAGKEGTTVFLVGGKPEV LAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASGAQIVTVAMGSPKQEIFMRDC RLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWLYRLLSQPSRIKRQLRLLRYLRW HYTGNL