Protein Info for KEDOAH_13545 in Escherichia coli ECRC99

Name: gppA
Annotation: guanosine-5'-triphosphate,3'-diphosphate diphosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 494 PF02541: Ppx-GppA" amino acids 21 to 301 (281 residues), 356.3 bits, see alignment E=1.3e-110 PF21447: Ppx-GppA_III" amino acids 308 to 479 (172 residues), 145.3 bits, see alignment E=1.7e-46

Best Hits

Swiss-Prot: 100% identical to GPPA_ECOBW: Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase (gppA) from Escherichia coli (strain K12 / MC4100 / BW2952)

KEGG orthology group: K01524, exopolyphosphatase / guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase [EC: 3.6.1.11 3.6.1.40] (inferred from 100% identity to eco:b3779)

MetaCyc: 100% identical to guanosine-5'-triphosphate,3'-diphosphate phosphatase (Escherichia coli K-12 substr. MG1655)
Guanosine-5'-triphosphate,3'-diphosphate diphosphatase. [EC: 3.6.1.40]

Predicted SEED Role

No annotation

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.11, 3.6.1.40

Use Curated BLAST to search for 3.6.1.11 or 3.6.1.40

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (494 amino acids)

>KEDOAH_13545 guanosine-5'-triphosphate,3'-diphosphate diphosphatase (Escherichia coli ECRC99)
MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAAGLNSENALSNEAMERG
WQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQEILGCPVQVISGEEEARLI
YQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSLSMGCVTWLERYFADRNLGQE
NFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQALQEIMMAQGMDERITLEKLQQLK
QRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIFTELNIQCMTLAGGALREGLVYGML
HLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVAANFFDQVENEWHLEAISRDLLISACQ
LHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPAQKKLLATLLLNQTNPVDLSSLHQQNAVP
PRVAEQLCRLLRLAIIFASRRRDDLVPEMTLQANHELLTLTLPQGWLTQHPLGKEIIAQE
SQWQSYVHWPLEVH