Protein Info for KEDOAH_12465 in Escherichia coli ECRC99

Name: rfaL
Annotation: O-antigen ligase RfaL

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 402 transmembrane" amino acids 14 to 32 (19 residues), see Phobius details amino acids 38 to 55 (18 residues), see Phobius details amino acids 67 to 85 (19 residues), see Phobius details amino acids 97 to 116 (20 residues), see Phobius details amino acids 128 to 147 (20 residues), see Phobius details amino acids 165 to 183 (19 residues), see Phobius details amino acids 190 to 225 (36 residues), see Phobius details amino acids 231 to 249 (19 residues), see Phobius details amino acids 321 to 343 (23 residues), see Phobius details amino acids 361 to 379 (19 residues), see Phobius details PF04932: Wzy_C" amino acids 195 to 337 (143 residues), 77.6 bits, see alignment E=4.4e-26

Best Hits

Swiss-Prot: 53% identical to RFAL_SALTY: O-antigen ligase (rfaL) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K02847, O-antigen ligase [EC: 6.-.-.-] (inferred from 100% identity to eoh:ECO103_4557)

MetaCyc: 53% identical to O-antigen ligase (Salmonella enterica enterica serovar Typhimurium str. LT2)
RXN1R65-45 [EC: 2.4.99.26]

Predicted SEED Role

"Oligosaccharide repeat unit polymerase Wzy; O-antigen ligase"

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.-.-.-

Use Curated BLAST to search for 2.4.99.26 or 6.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (402 amino acids)

>KEDOAH_12465 O-antigen ligase RfaL (Escherichia coli ECRC99)
MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVILMTITTIVYLCKRPKH
YLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAIIENTLLCTIAIPIILRDEK
REDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFADYRHRSISDSMVFLFPALLNL
WLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVIGLIWILMFKQWKLLLVGVMVAI
IALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGSALDLILENPVIGYGYGNVAYKDVY
NKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGLVSLMMLYCAILKECIKNGVKNKYRSP
YNAYYIILLSFIGYFVIRGNVEQIEPNLLGVYAGLLLAMKNK