Protein Info for KEDOAH_08620 in Escherichia coli ECRC99

Name: gcvT
Annotation: glycine cleavage system aminomethyltransferase GcvT

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00528: glycine cleavage system T protein
  • amino acids 2 to 359 (358 residues), 562 bits, E=2.5e-173
• PF01571: GCV_T
  • amino acids 7 to 254 (248 residues), 279 bits, E=3.2e-87
• PF08669: GCV_T_C
  • amino acids 280 to 357 (78 residues), 52.1 bits, E=4.9e-18

Best Hits

Swiss-Prot: 100% identical to GCST_ECO57: Aminomethyltransferase (gcvT) from Escherichia coli O157:H7

KEGG orthology group: K00605, aminomethyltransferase [EC: 2.1.2.10] (inferred from 100% identity to etw:ECSP_3872)

MetaCyc: 99% identical to aminomethyltransferase (Escherichia coli K-12 substr. MG1655)
Aminomethyltransferase. [EC: 1.4.1.27, 2.1.2.10]; 1.4.1.27 [EC: 1.4.1.27, 2.1.2.10]

Predicted SEED Role

"Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10)" in subsystem Glycine and Serine Utilization or Glycine cleavage system or Photorespiration (oxidative C2 cycle) (EC 2.1.2.10)

MetaCyc Pathways

• folate transformations III (E. coli) (9/9 steps found)
• folate transformations II (plants) (10/11 steps found)
• glycine biosynthesis II (3/3 steps found)
• glycine cleavage (3/3 steps found)
• glycine degradation (3/3 steps found)
• folate transformations I (9/13 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (6/9 steps found)
• photorespiration II (6/10 steps found)
• photorespiration I (5/9 steps found)
• photorespiration III (5/9 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism
• Nitrogen metabolism
• One carbon pool by folate

Isozymes

Compare fitness of predicted isozymes for: 2.1.2.10

Use Curated BLAST to search for 1.4.1.27 or 2.1.2.10

Protein Sequence (364 amino acids)

>KEDOAH_08620 glycine cleavage system aminomethyltransferase GcvT (Escherichia coli ECRC99)
MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHEVRTDAGMFDVSHMTIVDLRGS
RTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVYYFTEDFFRLVVNSATREK
DLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLFNDAQRQAVEGMKPFFGVQAG
DLFIATTGYTGEAGYEIALPNEKAADFWRALVEGGVKPCGLGARDTLRLEAGMNLYGQEM
DETISPLAANMGWTIAWEPADRDFIGREALEVQREHGTEKLVGLVMTEKGVLRNELPVRF
TDAQGNQHEGIITSGTFSPTLGYSIALARVPKGIGETAIVQIRNREMPVKVTKPVFVRNG
KAVA