Protein Info for KEDOAH_08555 in Escherichia coli ECRC99

Name: prfB
Annotation: peptide chain release factor 2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 293 TIGR00020: peptide chain release factor 2" amino acids 1 to 291 (291 residues), 524.2 bits, see alignment E=7.9e-162 PF03462: PCRF" amino acids 4 to 147 (144 residues), 167.7 bits, see alignment E=2.8e-53 PF00472: RF-1" amino acids 156 to 265 (110 residues), 146.1 bits, see alignment E=4.3e-47

Best Hits

Swiss-Prot: 100% identical to RF2_ECOLU: Peptide chain release factor 2 (prfB) from Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC)

KEGG orthology group: K02836, peptide chain release factor 2 (inferred from 100% identity to eco:b2891)

Predicted SEED Role

"Peptide chain release factor 2; programmed frameshift-containing"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (293 amino acids)

>KEDOAH_08555 peptide chain release factor 2 (Escherichia coli ECRC99)
MKQGLEDVSGLLELAVEADDEETFNEAVAELDALEEKLAQLEFRRMFSGEYDSADCYLDI
QAGSGGTEAQDWASMLERMYLRWAESRGFKTEIIEESEGEVAGIKSVTIKISGDYAYGWL
RTETGVHRLVRKSPFDSGGRRHTSFSSAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQ
HVNRTESAVRITHIPTGIVTQCQNDRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMED
NKSDIGWGSQIRSYVLDDSRIKDLRTGVETRNTQAVLDGSLDQFIEASLKAGL