Protein Info for KEDOAH_07890 in Escherichia coli ECRC99

Name: barA
Annotation: two-component sensor histidine kinase BarA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 918 transmembrane" amino acids 12 to 31 (20 residues), see Phobius details amino acids 177 to 199 (23 residues), see Phobius details PF09984: sCache_4" amino acids 31 to 177 (147 residues), 173.5 bits, see alignment E=7.1e-55 PF00672: HAMP" amino acids 198 to 248 (51 residues), 33.4 bits, see alignment 1.4e-11 PF00512: HisKA" amino acids 293 to 357 (65 residues), 79.3 bits, see alignment 5.2e-26 PF02518: HATPase_c" amino acids 404 to 518 (115 residues), 104.8 bits, see alignment E=1.1e-33 PF00072: Response_reg" amino acids 670 to 780 (111 residues), 104.2 bits, see alignment E=1.4e-33 PF01627: Hpt" amino acids 828 to 910 (83 residues), 54.8 bits, see alignment E=2.8e-18

Best Hits

Swiss-Prot: 100% identical to BARA_ECO57: Signal transduction histidine-protein kinase BarA (barA) from Escherichia coli O157:H7

KEGG orthology group: K07678, two-component system, NarL family, sensor histidine kinase BarA [EC: 2.7.13.3] (inferred from 100% identity to ecc:c3349)

MetaCyc: 100% identical to sensor histidine kinase BarA (Escherichia coli K-12 substr. MG1655)
Histidine kinase. [EC: 2.7.13.3]

Predicted SEED Role

"BarA sensory histidine kinase (= VarS = GacS)" in subsystem Type III secretion system orphans

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (918 amino acids)

>KEDOAH_07890 two-component sensor histidine kinase BarA (Escherichia coli ECRC99)
MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDAGASIIEPLAVSTEYGM
SLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFHLDPSSMQLGSNVPFPRQL
TVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYIALELDLKSVRLQQYKEIFIS
SVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRRGQLDSRVEGFMLGELDMLKNGI
NSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQNVELDLAKKRAQEAARIKSEFLANM
SHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTIERSANNLLAIINDVLDFSKLEAGKLI
LESIPFPLRSTLDEVVTLLAHSSHDKGLELTLNIKSDVPDNVIGDPLRLQQIITNLVGNA
IKFTENGNIDILVEKRALSNTKVQIEVQIRDTGIGIPERDQSRLFQAFRQADASISRRHG
GTGLGLVITQKLVNEMGGDISFHSQPNRGSTFWFHINLDLNPNIIIEGPSTQCLAGKRLA
YVEPNSAAAQCTLDILSETPLEVVYSPTFSALPPAHYDMMLLGIAVTFREPLTMQHERLA
KAVSMTDFLMLALPCHAQVNAEKLKQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPV
TDESKLAMTVMAVDDNPANLKLIGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQ
MPDMDGIRACELIHQLPHQQQTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNL
LLRYKPGSGISSRVVTPEVNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEV
RNKVEEQLVGENPEGLVDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELL
ELLDEMDNVAREASKILG