Protein Info for KEDOAH_07715 in Escherichia coli ECRC99

Name: cas1e
Annotation: type I-E CRISPR-associated endonuclease Cas1e

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 307 TIGR03638: CRISPR-associated endonuclease Cas1, subtype I-E/ECOLI" amino acids 8 to 272 (265 residues), 380.1 bits, see alignment E=5.3e-118 TIGR00287: CRISPR-associated endonuclease Cas1" amino acids 16 to 127 (112 residues), 74.6 bits, see alignment E=7.4e-25 amino acids 133 to 256 (124 residues), 103 bits, see alignment E=1.8e-33 PF01867: Cas_Cas1" amino acids 132 to 230 (99 residues), 35.1 bits, see alignment E=4.1e-13

Best Hits

Swiss-Prot: 84% identical to CAS1_ECOLI: CRISPR-associated endonuclease Cas1 (ygbT) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to ece:Z4064)

Predicted SEED Role

"CRISPR-associated protein Cas1" in subsystem CRISPRs

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (307 amino acids)

>KEDOAH_07715 type I-E CRISPR-associated endonuclease Cas1e (Escherichia coli ECRC99)
VTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHIPVGSVACIMLEPGTRV
SHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQAKLALTEDLRLKVVRKMY
ELRFREPPPARRSVDQLRGIEGSRVRQTYALLAKQYGVKWNGRKYDPKDWEKGDVVNRCI
SAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYDIADIIKFDSVVPKAFEIAARQP
AEPDKEVRLACRDIFRSTKLTGKLIPLIEKVLAAGEIEPPQPAPDMLPPAIPEPETLGDS
GHRGRGG