Protein Info for KEDOAH_07020 in Escherichia coli ECRC99

Name: dgcN
Annotation: diguanylate cyclase DgcN

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 408 transmembrane" amino acids 21 to 43 (23 residues), see Phobius details amino acids 155 to 177 (23 residues), see Phobius details PF17152: CHASE8" amino acids 45 to 146 (102 residues), 112.9 bits, see alignment E=7.6e-37 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 247 to 405 (159 residues), 116.2 bits, see alignment E=6.4e-38 PF00990: GGDEF" amino acids 249 to 403 (155 residues), 146 bits, see alignment E=8.5e-47

Best Hits

Swiss-Prot: 99% identical to DGCN_ECOLI: Diguanylate cyclase DgcN (dgcN) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to eok:G2583_3186)

MetaCyc: 99% identical to diguanylate cyclase DgcN (Escherichia coli K-12 substr. MG1655)
Diguanylate kinase. [EC: 2.7.7.65]

Predicted SEED Role

"Inner membrane protein YfiN"

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.65

Use Curated BLAST to search for 2.7.7.65

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (408 amino acids)

>KEDOAH_07020 diguanylate cyclase DgcN (Escherichia coli ECRC99)
MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLPLKQYAQKNLALTAATM
TYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILASWHYTRKDPGDTFSNFI
SHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAVLTGCILLASGIAITLTRH
LHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRFALDFNSLLDEMEEWQLRLQA
KNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSAARKTSALLFLDGDNFKYINDTWGHA
TGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMVLYGVQSESEVQQICSALTQIFNLPFD
LHNGHQTTMTLSIGYAMTIAHASAEKLQELADHNMYQAKHQRAEKLVR