Protein Info for JDDGAC_23720 in Escherichia coli ECRC98

Name: recD
Annotation: exodeoxyribonuclease V subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 608 PF21185: RecD_N" amino acids 7 to 108 (102 residues), 134.1 bits, see alignment E=9.7e-43 TIGR01447: exodeoxyribonuclease V, alpha subunit" amino acids 9 to 602 (594 residues), 656.1 bits, see alignment E=2.7e-201 PF13245: AAA_19" amino acids 154 to 304 (151 residues), 122.2 bits, see alignment E=8.7e-39 PF13604: AAA_30" amino acids 156 to 316 (161 residues), 97.7 bits, see alignment E=3.7e-31 PF00580: UvrD-helicase" amino acids 157 to 224 (68 residues), 30 bits, see alignment E=2.1e-10 PF13401: AAA_22" amino acids 163 to 297 (135 residues), 30.5 bits, see alignment E=2.1e-10 PF09848: SLFN-g3_helicase" amino acids 165 to 272 (108 residues), 21.2 bits, see alignment E=8e-08 PF05127: Helicase_RecD" amino acids 168 to 286 (119 residues), 54.8 bits, see alignment E=5.6e-18 PF01443: Viral_helicase1" amino acids 530 to 580 (51 residues), 25.8 bits, see alignment 4.6e-09 PF13538: UvrD_C_2" amino acids 534 to 579 (46 residues), 47.7 bits, see alignment 5.7e-16

Best Hits

Swiss-Prot: 99% identical to RECD_ECOLI: RecBCD enzyme subunit RecD (recD) from Escherichia coli (strain K12)

KEGG orthology group: K03581, exodeoxyribonuclease V alpha subunit [EC: 3.1.11.5] (inferred from 100% identity to ece:Z4136)

MetaCyc: 99% identical to exodeoxyribonuclease V subunit RecD (Escherichia coli K-12 substr. MG1655)
RXN0-4261 [EC: 5.6.2.3]; Exodeoxyribonuclease V. [EC: 5.6.2.3, 3.1.11.5]; RXN-19004 [EC: 5.6.2.3, 3.1.11.5]

Predicted SEED Role

"Exodeoxyribonuclease V alpha chain (EC 3.1.11.5)" in subsystem DNA-replication (EC 3.1.11.5)

Isozymes

Compare fitness of predicted isozymes for: 3.1.11.5

Use Curated BLAST to search for 3.1.11.5 or 5.6.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (608 amino acids)

>JDDGAC_23720 exodeoxyribonuclease V subunit alpha (Escherichia coli ECRC98)
MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSHDAGEGHVCLPLSRLEN
NEESHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMILCGDRLYLNRMWCNERTVA
RFFNEVNHAIEVDEALLAQTLDKLFPTGDEINWQKVAAAVALTRRISVISGGPGTGKTTT
VAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGKALRQLPLTDEQKKRIPEDASTL
HRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDLPMMSRLIDALPDHARVIFLGDRDQ
LASVEAGAVLGDICAYANAGFTAERAGQLSRLTGTHVPAGTGTEAASLRDSLCLLQKSYR
FGSDSGIGQLAAAINRGDKTAVKTVFQQDFTDIEKRLLQSGEDYIAMLEEALAGYGRYLD
LLQARAEPDLIIQAFNEYQLLCALREGPFGVAGLNERIEQFMQQKRKIHRNPHSRWYEGR
PVMIARNDSALGLFNGDIGIALDRGQGTRVWFAMPDGNIKSVQPSRLPEHETTWAMTVHK
SQGSEFDHAALILPSQRTPVVTRELVYTAVTRARRRLSLYADERILSAAIATRTERRSGL
AALFSSRE