Protein Info for JDDGAC_23295 in Escherichia coli ECRC98

Name: ygfK
Annotation: putative selenate reductase subunit YgfK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1032 TIGR03315: putative selenate reductase, YgfK subunit" amino acids 2 to 1027 (1026 residues), 1785.1 bits, see alignment E=0 PF14691: Fer4_20" amino acids 453 to 532 (80 residues), 59.4 bits, see alignment 1.2e-19 PF07992: Pyr_redox_2" amino acids 552 to 839 (288 residues), 146.4 bits, see alignment E=4.9e-46 PF01494: FAD_binding_3" amino acids 552 to 584 (33 residues), 23.1 bits, see alignment (E = 1.7e-08) PF00890: FAD_binding_2" amino acids 553 to 587 (35 residues), 22.6 bits, see alignment (E = 2.3e-08) PF13450: NAD_binding_8" amino acids 555 to 592 (38 residues), 34.8 bits, see alignment (E = 6.5e-12) PF13738: Pyr_redox_3" amino acids 636 to 824 (189 residues), 32.2 bits, see alignment E=2.7e-11

Best Hits

Swiss-Prot: 100% identical to YGFK_ECO57: Putative oxidoreductase YgfK (ygfK) from Escherichia coli O157:H7

KEGG orthology group: K12527, putative selenate reductase [EC: 1.97.1.9] (inferred from 100% identity to eok:G2583_3532)

Predicted SEED Role

"Predicted oxidoreductase, Fe-S subunit"

Isozymes

Compare fitness of predicted isozymes for: 1.97.1.9

Use Curated BLAST to search for 1.97.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1032 amino acids)

>JDDGAC_23295 putative selenate reductase subunit YgfK (Escherichia coli ECRC98)
MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTVSVFGETCATPVGPAAG
PHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAEDECFNTEWSTEFTLLKAWD
EYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQPPMQQFIDNMMDASDHPKFA
QYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTSMVHGVTLSTMHGCPPHEIEAIC
RYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGYIGLKEESFDHDLKLTQALEMLERL
MALAKEKSLGFGVKLTNTLGTINNKGALPGEEMYMSGRALFPLSINVAAVLSRAFDGKLP
ISYSGGASQLTIRDIFDTGIRPITMATDLLKPGGYLRLSACMRELEGSDAWGLDHVDVER
LNRLAADALTMEYTQKHWKPEERIEVAEDLPLTDCYVAPCVTACAIKQDIPEYIRLLGEH
RYADALELIYQRNALPAITGHICDHQCQYNCTRLDYDSALNIRELKKVALEKGWDEYKQR
WHKPAGSGSRHPVAVIGAGPAGLAAGYFLARAGHPVTLFEREANAGGVVKNIIPQFRIPA
ELIQHDIDFVAAHGVKFEYGCSPDLTVEQLKNQDFHYVLIATGTDKNSGVKLAGDNQNVL
KSLPFLREYNKGTALKLGKHVVVVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAW
REEYEEALHDGVEFRFLNNPERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDS
LITAIGEQQDTEALNAMGVPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTA
RRATDAILSRENIRSHQNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLE
CNYVCSKCVDVCPNRANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKIT
VFSLAQDFDNSSNPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVH
QHHHYLLGRVEV