Protein Info for JDDGAC_21395 in Escherichia coli ECRC98

Name: aaeA
Annotation: p-hydroxybenzoic acid efflux pump subunit AaeA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 309 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details PF00529: CusB_dom_1" amino acids 22 to 284 (263 residues), 45.1 bits, see alignment E=1.6e-15 PF16576: HlyD_D23" amino acids 46 to 237 (192 residues), 56.6 bits, see alignment E=4.5e-19 TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 46 to 288 (243 residues), 108.4 bits, see alignment E=1.8e-35 PF13533: Biotin_lipoyl_2" amino acids 47 to 93 (47 residues), 57.3 bits, see alignment 2.1e-19 PF13437: HlyD_3" amino acids 155 to 238 (84 residues), 40.7 bits, see alignment E=6.6e-14

Best Hits

Swiss-Prot: 100% identical to AAEA_ECO57: p-hydroxybenzoic acid efflux pump subunit AaeA (aaeA) from Escherichia coli O157:H7

KEGG orthology group: None (inferred from 100% identity to etw:ECSP_4210)

MetaCyc: 99% identical to aromatic carboxylic acid efflux pump membrane fusion protein (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-233

Predicted SEED Role

"Membrane fusion component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (309 amino acids)

>JDDGAC_21395 p-hydroxybenzoic acid efflux pump subunit AaeA (Escherichia coli ECRC98)
VKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFSADVVAIAPDVSGLITQ
VNVHNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQEKRQEAGRRNRLGVQAMSR
EEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAPADGWVTNLNVYTGEFITRGST
AVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGSNKVLKGTVDSVAAGVTNASSTRD
DKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWPAGTTATVVVTGKQDRDESQDSFFRK
MAHRLREFG