Protein Info for JDDGAC_20050 in Escherichia coli ECRC98

Name: rsmJ
Annotation: 16S rRNA (guanine(1516)-N(2))-methyltransferase RsmJ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 PF04445: SAM_MT" amino acids 19 to 246 (228 residues), 348.5 bits, see alignment E=7.7e-109

Best Hits

Swiss-Prot: 99% identical to RSMJ_ECOLC: Ribosomal RNA small subunit methyltransferase J (rsmJ) from Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks)

KEGG orthology group: None (inferred from 99% identity to eco:b3497)

MetaCyc: 99% identical to 16S rRNA m2G1516 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN0-6731 [EC: 2.1.1.242]

Predicted SEED Role

"rRNA small subunit methyltransferase J"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.242

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (250 amino acids)

>JDDGAC_20050 16S rRNA (guanine(1516)-N(2))-methyltransferase RsmJ (Escherichia coli ECRC98)
VKICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLELRKRDEPKLGGIFVDFV
GGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRDAFVLASVGCRVRMLERNP
VVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTDITPRPQVVYLDPMFPHKQKS
ALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVKRPDYAPPLANVSTPNAVVTKGH
RFDIYAGTPV