Protein Info for JDDGAC_17990 in Escherichia coli ECRC98

Name: pldA
Annotation: phospholipase A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02253: PLA1
  • amino acids 43 to 285 (243 residues), 300.9 bits, E=4.5e-94

Best Hits

Swiss-Prot: 100% identical to PA1_SHIFL: Phospholipase A1 (pldA) from Shigella flexneri

KEGG orthology group: K01058, phospholipase A1 [EC: 3.1.1.32] (inferred from 100% identity to eco:b3821)

MetaCyc: 100% identical to outer membrane phospholipase A (Escherichia coli K-12 substr. MG1655)
Lysophospholipase. [EC: 3.1.1.5]; 3.1.1.5 [EC: 3.1.1.5]; Phospholipase A(2). [EC: 3.1.1.5, 3.1.1.4]; Phospholipase A(1). [EC: 3.1.1.5, 3.1.1.4, 3.1.1.32]

Predicted SEED Role

"Phospholipase A1 precursor (EC 3.1.1.32, EC 3.1.1.4); Outer membrane phospholipase A"

MetaCyc Pathways

• phospholipid remodeling (phosphatidate, yeast) (1/2 steps found)
• phosphatidylcholine acyl editing (2/4 steps found)
• phospholipid remodeling (phosphatidylethanolamine, yeast) (2/4 steps found)
• phospholipases (1/5 steps found)
• anandamide biosynthesis II (2/8 steps found)
• anandamide biosynthesis I (4/12 steps found)
• peptido-conjugates in tissue regeneration biosynthesis (3/17 steps found)

KEGG Metabolic Maps

• Arachidonic acid metabolism
• Ether lipid metabolism
• Glycerophospholipid metabolism
• Linoleic acid metabolism
• alpha-Linolenic acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.5

Use Curated BLAST to search for 3.1.1.32 or 3.1.1.4 or 3.1.1.5

Protein Sequence (289 amino acids)

>JDDGAC_17990 phospholipase A (Escherichia coli ECRC98)
MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQEHDNPFTLYPYDTNYL
IYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGILGPNSVLGASYTQKSWWQL
SNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGYNHDSNGRSDPTSRSWNRLYT
RLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLKIGYHLGDAVLSAKGQYNWNTGY
GGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQTRVGVGVMLNDLF