Protein Info for JDDGAC_14115 in Escherichia coli ECRC98

Name: ampE
Annotation: beta-lactamase regulator AmpE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 286 signal peptide" amino acids 1 to 17 (17 residues), see Phobius details transmembrane" amino acids 40 to 58 (19 residues), see Phobius details amino acids 65 to 86 (22 residues), see Phobius details amino acids 144 to 168 (25 residues), see Phobius details amino acids 266 to 285 (20 residues), see Phobius details PF17113: AmpE" amino acids 1 to 286 (286 residues), 526 bits, see alignment E=1.2e-162

Best Hits

Swiss-Prot: 99% identical to AMPE_ECOLI: Protein AmpE (ampE) from Escherichia coli (strain K12)

KEGG orthology group: K03807, AmpE protein (inferred from 99% identity to eco:b0111)

Predicted SEED Role

"AmpE protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (286 amino acids)

>JDDGAC_14115 beta-lactamase regulator AmpE (Escherichia coli ECRC98)
MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTLGMTIIAMGVTFLLLRA
LQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRNDSHARATMAGELTMIHGVP
AGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPVTLMGYAFLRAWQYWLARYQT
PHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKALPAWFASLGDFRTSQYQVLTRLA
QFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVVVIALLTIYGALV