Protein Info for JDDGAC_12180 in Escherichia coli ECRC98

Name: malZ
Annotation: maltodextrin glucosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 605 PF02903: Alpha-amylase_N" amino acids 3 to 105 (103 residues), 31.6 bits, see alignment E=1.6e-11 PF00128: Alpha-amylase" amino acids 178 to 494 (317 residues), 297 bits, see alignment E=2.6e-92

Best Hits

Swiss-Prot: 99% identical to MALZ_ECOLI: Maltodextrin glucosidase (malZ) from Escherichia coli (strain K12)

KEGG orthology group: K01187, alpha-glucosidase [EC: 3.2.1.20] (inferred from 100% identity to ecf:ECH74115_0480)

MetaCyc: 99% identical to maltodextrin glucosidase (Escherichia coli K-12 substr. MG1655)
Alpha-glucosidase. [EC: 3.2.1.20]; 3.2.1.20 [EC: 3.2.1.20]; 3.2.1.20 [EC: 3.2.1.20]; 3.2.1.20 [EC: 3.2.1.20]; 3.2.1.20 [EC: 3.2.1.20]

Predicted SEED Role

"Maltodextrin glucosidase (EC 3.2.1.20)" in subsystem Maltose and Maltodextrin Utilization (EC 3.2.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (605 amino acids)

>JDDGAC_12180 maltodextrin glucosidase (Escherichia coli ECRC98)
MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDNEEMSVPMHKQRSQPQP
GVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMPPARLEQFAVDVPDIGPQW
AADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILRDWDEPVTAQAGGSTFYGGDL
DGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYRHVDPQFGGDGALLRLRHNTQQL
GMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPESPWRDWYSFSDDGTALDWLGYASLP
KLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGWRLDVVHMLGEAGGARNNLQHVAGITE
AAKETQPEAYIVGEHFGDARQWLQADVEDAAMNYRGFTFPLWGFLANTDISYDPQQIDAQ
TCMAWMDNYRAGLSHQQQLRMFNQLDSHDTARFKTLLGREVARLPLAVVWLFTWPGVPCI
YYGDEVGLDGKNDPFCRKPFPWQVEKQDMALFALYQRMIALRKKSQALRRGGCQVLYAED
NVVVFVRVLNQQRVLVAINRGEACEVVLPASPFLNVVQWQRKEGHGQLTDGILALPAISA
TVWMN