Protein Info for JDDGAC_09915 in Escherichia coli ECRC98

Name: dinG
Annotation: ATP-dependent DNA helicase DinG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 716 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details PF00270: DEAD" amino acids 31 to 97 (67 residues), 27 bits, see alignment E=7e-10 PF06733: DEAD_2" amino acids 198 to 264 (67 residues), 31.2 bits, see alignment E=3.4e-11 PF13307: Helicase_C_2" amino acids 529 to 688 (160 residues), 123.8 bits, see alignment E=1.5e-39

Best Hits

Swiss-Prot: 100% identical to DING_ECO57: ATP-dependent DNA helicase DinG (dinG) from Escherichia coli O157:H7

KEGG orthology group: K03722, ATP-dependent DNA helicase DinG [EC: 3.6.4.12] (inferred from 100% identity to eco:b0799)

MetaCyc: 100% identical to ATP-dependent DNA helicase DinG (Escherichia coli K-12 substr. MG1655)
RXN0-4261 [EC: 5.6.2.3]

Predicted SEED Role

"ATP-dependent helicase DinG/Rad3" in subsystem DNA repair, bacterial DinG and relatives

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12 or 5.6.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (716 amino acids)

>JDDGAC_09915 ATP-dependent DNA helicase DinG (Escherichia coli ECRC98)
MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAGEEGRHLAIEAPTGVGK
TLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKKIIPDLKFTAAFGRGRYVC
PRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLKGDLDTYKWDGLRDHTDIAID
DDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAEVVVANHALVMAAMESEAVLPDP
KNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQLDLFTKLVATCMEQFRPKTIPPLAI
PERLNAHCEELYELIASLNNILNLYMPAGQEAEHRFAMGELPDEVLEICQRLAKLTEMLR
GLAELFLNDLSEKTGSHDIVRLHRLILQMNRALGMFEAQSKLWRLASLAQSSGAPVTKWA
TREEREGQLHLWFHCVGIRVSDQLERLLWRSIPHIIVTSATLRSLNSFSRLQEMSGLKEK
AGDRFVALDSPFNHCEQGKIVIPRMRVEPSIDNEEQHIAEMAAFFREQVESKKHLGMLVL
FASGRAMQRFLDYVTDLRLMLLVQGDQPRYRLVELHRKRVANGERSVLVGLQSFAEGLDL
KGDMLSQVHIHKIAFPPIDSPVVITEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRS
HGCWGEVVIYDKRLLTKNYGKRLLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR