Protein Info for IAI46_25165 in Serratia liquefaciens MT49

Annotation: bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01173: UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase
  • amino acids 6 to 455 (450 residues), 674.1 bits, E=5e-207
• PF01128: IspD
  • amino acids 6 to 68 (63 residues), 28.1 bits, E=4.3e-10
• PF00483: NTP_transferase
  • amino acids 8 to 212 (205 residues), 48.7 bits, E=2e-16
• PF12804: NTP_transf_3
  • amino acids 8 to 121 (114 residues), 75.6 bits, E=1.3e-24
• PF00132: Hexapep
  • amino acids 268 to 300 (33 residues), 27.6 bits, see alignment (E = 4.2e-10)
  • amino acids 395 to 429 (35 residues), 32.1 bits, 1.6e-11
• PF14602: Hexapep_2
  • amino acids 276 to 298 (23 residues), 14 bits, see alignment (E = 8.7e-06)
  • amino acids 395 to 429 (35 residues), 30.4 bits, 6.5e-11

Best Hits

Swiss-Prot: 98% identical to GLMU_SERP5: Bifunctional protein GlmU (glmU) from Serratia proteamaculans (strain 568)

KEGG orthology group: K04042, bifunctional UDP-N-acetylglucosamine pyrophosphorylase / Glucosamine-1-phosphate N-acetyltransferase [EC: 2.3.1.157 2.7.7.23] (inferred from 98% identity to srs:SerAS12_5006)

MetaCyc: 81% identical to fused N-acetylglucosamine-1-phosphate uridyltransferase and glucosamine-1-phosphate acetyltransferase (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylglucosamine diphosphorylase. [EC: 2.7.7.23]; Glucosamine-1-phosphate N-acetyltransferase. [EC: 2.7.7.23, 2.3.1.157]

Predicted SEED Role

"N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)" in subsystem Peptidoglycan Biosynthesis or Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.3.1.157, EC 2.7.7.23)

MetaCyc Pathways

• peptidoglycan recycling I (13/14 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• O-antigen building blocks biosynthesis (E. coli) (9/11 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (5/7 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• chitin biosynthesis (5/9 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (7/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.157 or 2.7.7.23

Protein Sequence (456 amino acids)

>IAI46_25165 bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU (Serratia liquefaciens MT49)
MSNSAMSVVILAAGKGTRMYSDLPKVLHPLAGKPMVQHVIDAAMKLGAQNVHLVYGHGGE
LLKKTLTDSALNWVLQAEQLGTGHAMQQAAPHFADDEDVLMLYGDVPLISVDTLQRLLAA
KPQGGIGLLTVKLADPSGYGRIVREQDTVVGIVEHKDASEAQRQINEINTGILVANGRDL
KRWLGMLNNDNAQGEFYITDIIALAHADGKKIEAVHPSRLSEVEGVNNRLQLSRLERIYQ
AEQSEKLLLAGVMLLDPARFDLRGELVHGRDISIDANVIIEGSVKLGDRVKIGAGCVLKN
CVIGDDCEISPYSVLEDAVLAAECTVGPFARLRPGAELAVGAHVGNFVEMKKARLGKGSK
AGHLSYLGDAEIGDDVNIGAGTITCNYDGANKHKTIIGDGVFVGSDTQLVAPVSVGKGST
IAAGTTVTRDIGEDELVLSRVKQVHIQGWQRPVKKR