Protein Info for IAI46_24955 in Serratia liquefaciens MT49

Annotation: nitrogen regulation protein NR(II)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 349 PF00989: PAS" amino acids 11 to 98 (88 residues), 30.3 bits, see alignment E=5.4e-11 PF00512: HisKA" amino acids 131 to 187 (57 residues), 54.9 bits, see alignment E=1.1e-18 PF02518: HATPase_c" amino acids 234 to 348 (115 residues), 68.9 bits, see alignment E=7.7e-23

Best Hits

Swiss-Prot: 87% identical to NTRB_KLEOX: Sensory histidine kinase/phosphatase NtrB (ntrB) from Klebsiella oxytoca

KEGG orthology group: None (inferred from 97% identity to srr:SerAS9_4967)

MetaCyc: 86% identical to protein histidine kinase NtrB (Escherichia coli K-12 substr. MG1655)
Histidine kinase. [EC: 2.7.13.3]

Predicted SEED Role

"Nitrogen regulation protein NtrB (EC 2.7.13.3)" (EC 2.7.13.3)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (349 amino acids)

>IAI46_24955 nitrogen regulation protein NR(II) (Serratia liquefaciens MT49)
MATGKLPDAGQILNSLINSILLLDDSLAVHYANPAAQQLLAQSSRKLFGTPLPDLLGYFS
LNVDLMRESLHAGQGFTDNEVTLVVDGRAHILSLTAQALPDGYILIELAPMDNQRRLSQE
QLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLAKALPDPSLTEYTKVIIEQADRLRN
LVDRLLGPQRPGQHITQSIHQVAERVCQLVSLEKPDNVTLVRDYDPSLPEMAHDPDQIEQ
VLLNITRNALQALGETGGTITLRTRTAFQITLHGTRYRLAARIDVEDDGPGVPAQLQDTL
FYPMVSGREGGTGLGLSIARNLIDQHCGKIEFNSWPGHTEFSVYLPIRQ