Protein Info for IAI46_24950 in Serratia liquefaciens MT49

Annotation: glutamate--ammonia ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00653: glutamine synthetase, type I
  • amino acids 4 to 468 (465 residues), 709.1 bits, E=1.1e-217
• PF03951: Gln-synt_N
  • amino acids 14 to 95 (82 residues), 113.9 bits, E=2.2e-37
• PF00120: Gln-synt_C
  • amino acids 103 to 466 (364 residues), 465.1 bits, E=1.4e-143

Best Hits

Swiss-Prot: 92% identical to GLN1B_ECOLI: Glutamine synthetase (glnA) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to srr:SerAS9_4966)

MetaCyc: 92% identical to glutamine synthetase (Escherichia coli K-12 substr. MG1655)
Glutamate--ammonia ligase. [EC: 6.3.1.2]

Predicted SEED Role

"Glutamine synthetase type I (EC 6.3.1.2)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis or Glutamine synthetases or Peptidoglycan Biosynthesis (EC 6.3.1.2)

MetaCyc Pathways

• L-glutamate and L-glutamine biosynthesis (7/7 steps found)
• L-arginine biosynthesis II (acetyl cycle) (9/10 steps found)
• L-aspartate degradation II (aerobic) (3/3 steps found)
• L-aspartate degradation III (anaerobic) (3/3 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• superpathway of ammonia assimilation (plants) (3/3 steps found)
• ammonia assimilation cycle I (2/2 steps found)
• ammonia assimilation cycle II (2/2 steps found)
• L-glutamine biosynthesis I (1/1 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.1.2

Protein Sequence (469 amino acids)

>IAI46_24950 glutamate--ammonia ligase (Serratia liquefaciens MT49)
MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNADFFEEGKMFDGSSIGGWKG
INESDMVLMPDASTAVLDPFFEESTLIIRCDILEPGTMQGYDRDPRSISKRAEDFLRSSG
IADTVLFGPEPEFFLFDDIRFGSSIRGSHVAIDDIEGAWNSGTQYDGGNKGHRPAVKGGY
FPVPPVDSSQDIRSTMCLTMEEMGLVVEAHHHEVATAGQNEVATRFNTMTKKADEIQIYK
YVVHNVAHAFGKTATFMPKPMFGDNGSGMHCHMSLSKNGTNLFAGDKYGGLSETALFYIG
GIIKHAKAINALANPTTNSYKRLVPGYEAPVMLAYSARNRSASIRIPVVASPKARRIEAR
FPDPAANPYLCFAALLMAGLDGVINKIHPGDAMDKNLYDLPPEEEAEIPKVAGSLDEALA
ALSEDREFLTRGGVFTDDAIDAYIDLRKEEMDRVRMTPHPVEFELYYSV