Protein Info for IAI46_24895 in Serratia liquefaciens MT49

Annotation: bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 703 TIGR00691: RelA/SpoT family protein" amino acids 26 to 700 (675 residues), 945.4 bits, see alignment E=9.2e-289 PF13328: HD_4" amino acids 26 to 175 (150 residues), 185.6 bits, see alignment E=1.5e-58 PF01966: HD" amino acids 45 to 144 (100 residues), 49.2 bits, see alignment E=1.8e-16 PF04607: RelA_SpoT" amino acids 235 to 345 (111 residues), 143.5 bits, see alignment E=9.5e-46 PF02824: TGS" amino acids 390 to 449 (60 residues), 74.4 bits, see alignment 1.8e-24 PF19296: RelA_AH_RIS" amino acids 461 to 547 (87 residues), 36.8 bits, see alignment E=1.4e-12 PF13291: ACT_4" amino acids 624 to 700 (77 residues), 59 bits, see alignment E=1.8e-19

Best Hits

Swiss-Prot: 92% identical to SPOT_ECOLI: Bifunctional (p)ppGpp synthase/hydrolase SpoT (spoT) from Escherichia coli (strain K12)

KEGG orthology group: K01139, guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase [EC: 3.1.7.2] (inferred from 92% identity to dze:Dd1591_4147)

MetaCyc: 92% identical to bifunctional (p)ppGpp synthase/hydrolase SpoT (Escherichia coli K-12 substr. MG1655)
Guanosine-3',5'-bis(diphosphate) 3'-diphosphatase. [EC: 3.1.7.2]; 3.1.7.2 [EC: 3.1.7.2]; GTP diphosphokinase. [EC: 3.1.7.2, 2.7.6.5]

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5, EC 3.1.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.5

Use Curated BLAST to search for 2.7.6.5 or 3.1.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (703 amino acids)

>IAI46_24895 bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase (Serratia liquefaciens MT49)
MYLFESLNLLIQRYLPEEQIKRLKQAYLVARDAHEGQTRSSGEPYITHPVAVACILAEMR
LDHETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSKLDKLKFQDKKEAQAENFR
KMIMAMVQDIRVVLIKLADRTHNMRTLGSLRPDKRRRIARETLEIYSPLAHRLGIHHLKT
ELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILAEIEGRLTEAGIPCRVSGREKHL
YSIYLKMHLKEQRFHSIMDIYAFRVIVKEVDTCYRVLGQAHSLYKPRPGRVKDYIAIPKA
NGYQSLHTSLIGPHGVPVEVQIRTEDMDQMAEMGVAAHWAYKEKEQGETGTTAQIRAQRW
MQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFTPEGRIVELPAGATPVDFAYAVHTDIG
HACVGARVDRQPYPLSQSLTSGQTVEIITAPGARPNAAWLNFVVSSKARAKIRQMLKNLK
RDDSVGLGRRLLNHALGGSRKLAEIPPENIQHELDRMKLATLDDLLAEIGLGNAMSVVVA
KNLQGDQSNLGTSSGVRNLAIKGADGVLITFAKCCRPIPGDPIIAHVSPGKGLVIHHESC
RNIRGYQKEPEKFMAVEWDKDIEQEFIAEIKVDMFNHQGALANLTAAINAAESNIQSLNT
EEKDGRVYSAFIRLTTRDRIHLANIMRKIRIMPDVIKVNRNRN