Protein Info for IAI46_24845 in Serratia liquefaciens MT49

Annotation: orotate phosphoribosyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00336: orotate phosphoribosyltransferase
  • amino acids 10 to 187 (178 residues), 227.4 bits, E=4.9e-72
• PF00156: Pribosyltran
  • amino acids 50 to 163 (114 residues), 39.3 bits, E=2e-14

Best Hits

Swiss-Prot: 99% identical to PYRE_SERP5: Orotate phosphoribosyltransferase (pyrE) from Serratia proteamaculans (strain 568)

KEGG orthology group: K00762, orotate phosphoribosyltransferase [EC: 2.4.2.10] (inferred from 99% identity to spe:Spro_4846)

MetaCyc: 90% identical to orotate phosphoribosyltransferase (Escherichia coli K-12 substr. MG1655)
Orotate phosphoribosyltransferase. [EC: 2.4.2.10]

Predicted SEED Role

"Orotate phosphoribosyltransferase (EC 2.4.2.10)" in subsystem De Novo Pyrimidine Synthesis (EC 2.4.2.10)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• UMP biosynthesis I (6/6 steps found)
• UMP biosynthesis II (6/6 steps found)
• UMP biosynthesis III (5/6 steps found)

KEGG Metabolic Maps

• Drug metabolism - other enzymes
• Pyrimidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.2.10

Protein Sequence (213 amino acids)

>IAI46_24845 orotate phosphoribosyltransferase (Serratia liquefaciens MT49)
MKAYQRQFIEFALNKQVLKFGEFTLKSGRTSPYFFNAGLFNTGRDLALLGRFYAEALMDS
GIEFDLLFGPAYKGIPIATTTAVALAEHHERDVPYCFNRKEAKTHGEGGTLVGSPLQGRV
MLVDDVITAGTAIRESMEIIGASGATLAGVLISLDRQERGRADISAIQEVERDYHCKVIS
IVTLKDLIVYLEEKPEMADHLAAVRAYREQYGV