Protein Info for IAI46_24795 in Serratia liquefaciens MT49

Annotation: lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04413: Glycos_transf_N
  • amino acids 33 to 211 (179 residues), 234 bits, E=8.9e-74
• PF00534: Glycos_transf_1
  • amino acids 244 to 398 (155 residues), 33.9 bits, E=2.3e-12

Best Hits

Swiss-Prot: 81% identical to KDTA_ECO57: 3-deoxy-D-manno-octulosonic acid transferase (waaA) from Escherichia coli O157:H7

KEGG orthology group: K02527, 3-deoxy-D-manno-octulosonic-acid transferase [EC: 2.-.-.-] (inferred from 99% identity to spe:Spro_4836)

MetaCyc: 81% identical to KDO transferase (Escherichia coli K-12 substr. MG1655)
KDOTRANS2-RXN [EC: 2.4.99.13]; KDOTRANS-RXN [EC: 2.4.99.13, 2.4.99.12]

Predicted SEED Role

"Lipid IVA 3-deoxy-D-manno-octulosonic acid transferase (EC 2.4.99.12) [often with (EC 2.4.99.13) also]" (EC 2.4.99.12, EC 2.4.99.13)

MetaCyc Pathways

• superpathway of (Kdo)₂-lipid A biosynthesis (17/17 steps found)
• Kdo transfer to lipid IV_A (Brucella) (2/2 steps found)
• Kdo transfer to lipid IV_A (E. coli) (2/2 steps found)
• Kdo transfer to lipid IV_A (H. pylori) (2/2 steps found)
• Kdo transfer to lipid IV_A (P. gingivalis) (2/2 steps found)
• Kdo transfer to lipid IV_A (P. putida) (2/2 steps found)
• Kdo transfer to lipid IV_A (generic) (2/2 steps found)
• superpathway of Kdo₂-lipid A biosynthesis (18/25 steps found)
• Kdo transfer to lipid IV_A (Haemophilus) (1/2 steps found)
• Kdo transfer to lipid IV_A (Vibrio cholerae serogroup O1 El Tor) (1/2 steps found)
• Kdo transfer to lipid IV_A (Chlamydia) (2/5 steps found)

KEGG Metabolic Maps

• Lipopolysaccharide biosynthesis
• Puromycin biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.-.-.-

Use Curated BLAST to search for 2.-.-.- or 2.4.99.12 or 2.4.99.13

Protein Sequence (425 amino acids)

>IAI46_24795 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase (Serratia liquefaciens MT49)
MLLRLYQVLLYLIQPLIWLRLLLRSRKAPAYRKRWAERYGFCAGKVVPGGIMLHSVSVGE
TLAAIPLVRALRHRYPYLPITVTTMTPTGSERVQSAFGKDVHHVYLPYDLPGSMRRFLDQ
VNPKLVIIMETELWPNLINALHQRQIPLVIANARLSARSAAGYKKIGGFVRDMLRRITLI
AAQNQEDGERFIELGLKRSQLTVTGSLKFDISVTPELAARAVTLRRQWAPRRPVWIATST
HEGEESILLAAHRKLLEKHPDLLLILVPRHPERFATAKDLVQKAGFSYTLRSSGEIPSGG
TQVVIGDTMGELMLLYGIADLAFVGGSLVERGGHNPLEAAAHAIPVLMGPHTFNFKDICA
KLSQAEGLITVTDEESLVKEVATLLTDEDYRRYYGRHAVEVLYQNQGALQRLLQLLEPHL
PPRSH