Protein Info for IAI46_24470 in Serratia liquefaciens MT49

Annotation: Si-specific NAD(P)(+) transhydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 465 PF00890: FAD_binding_2" amino acids 8 to 48 (41 residues), 21.8 bits, see alignment 5.1e-08 PF07992: Pyr_redox_2" amino acids 8 to 325 (318 residues), 216.9 bits, see alignment E=2.1e-67 PF12831: FAD_oxidored" amino acids 8 to 53 (46 residues), 31 bits, see alignment 9e-11 PF13450: NAD_binding_8" amino acids 11 to 62 (52 residues), 25.3 bits, see alignment 7.4e-09 PF00070: Pyr_redox" amino acids 177 to 254 (78 residues), 63.7 bits, see alignment E=9.3e-21 PF02852: Pyr_redox_dim" amino acids 345 to 456 (112 residues), 92.7 bits, see alignment E=8.6e-30

Best Hits

Swiss-Prot: 99% identical to STHA_SERP5: Soluble pyridine nucleotide transhydrogenase (sthA) from Serratia proteamaculans (strain 568)

KEGG orthology group: K00322, NAD(P) transhydrogenase [EC: 1.6.1.1] (inferred from 99% identity to srs:SerAS12_4869)

MetaCyc: 84% identical to soluble pyridine nucleotide transhydrogenase (Escherichia coli K-12 substr. MG1655)
NAD(P)H oxidase. [EC: 1.6.3.1]; 1.6.3.- [EC: 1.6.3.1]; NAD(P)(+) transhydrogenase (B-specific). [EC: 1.6.3.1, 1.6.1.1, 1.6.1.3]

Predicted SEED Role

"Soluble pyridine nucleotide transhydrogenase (EC 1.6.1.1)" in subsystem Phosphate metabolism (EC 1.6.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.6.1.1 or 1.6.1.3 or 1.6.3.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (465 amino acids)

>IAI46_24470 Si-specific NAD(P)(+) transhydrogenase (Serratia liquefaciens MT49)
MQQHYQFDAIVIGSGPGGEGAAMGLVKQGARVAVIERYNNVGGGCTHWGTIPSKALRHAV
SRIIEFNQNPLYNNSRTLSATFPDILRHADNVINQQTRMRQGFYERNQCKLFAGDARFID
ANTVSVSYMDGTQDTIRADHIVIACGSRPYNPSSVDFNHPRIYNSDSILELNHEPRHVII
YGAGVIGCEYASIFRGLNVKVDLINTRDRLLAFLDQEMSDSLSYHFWNNGVVIRHNEEFE
KIEGTEDGVIVHLKSGKKVKADCLLYANGRTGNTDSLGLENVGLESDSRGLLKVNSMYQT
ALPHIYAVGDVIGYPSLASAAYDQGRIAAQAISSGEASGHLIEDIPTGIYTIPEISSVGK
TEQELTAMKVPYEVGRAQFKHLARAQIAGMNVGSLKILFHRDTLQILGIHCFGERAAEII
HIGQAIMEQKGEGNTIEYFVNTTFNYPTMAEAYRVAALNGLNRLF