Protein Info for IAI46_23655 in Serratia liquefaciens MT49

Annotation: nitrite reductase large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 849 PF07992: Pyr_redox_2" amino acids 5 to 289 (285 residues), 188.6 bits, see alignment E=6.3e-59 TIGR02374: nitrite reductase [NAD(P)H], large subunit" amino acids 6 to 800 (795 residues), 1271.8 bits, see alignment E=0 PF13738: Pyr_redox_3" amino acids 75 to 280 (206 residues), 36.8 bits, see alignment E=1e-12 PF00070: Pyr_redox" amino acids 148 to 226 (79 residues), 62.5 bits, see alignment E=1.5e-20 PF18267: Rubredoxin_C" amino acids 321 to 384 (64 residues), 37.8 bits, see alignment 5.2e-13 PF04324: Fer2_BFD" amino acids 423 to 470 (48 residues), 56.3 bits, see alignment 1.1e-18 PF03460: NIR_SIR_ferr" amino acids 559 to 621 (63 residues), 42.1 bits, see alignment 2.1e-14 PF01077: NIR_SIR" amino acids 632 to 769 (138 residues), 64.6 bits, see alignment E=2.6e-21

Best Hits

Swiss-Prot: 84% identical to NIRB_ECOLI: Nitrite reductase (NADH) large subunit (nirB) from Escherichia coli (strain K12)

KEGG orthology group: K00362, nitrite reductase (NAD(P)H) large subunit [EC: 1.7.1.4] (inferred from 85% identity to cro:ROD_44441)

MetaCyc: 84% identical to nitrite reductase (NADH) large subunit (Escherichia coli K-12 substr. MG1655)
RXN-13854 [EC: 1.7.1.15]

Predicted SEED Role

"Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.1.4

Use Curated BLAST to search for 1.7.1.15 or 1.7.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (849 amino acids)

>IAI46_23655 nitrite reductase large subunit (Serratia liquefaciens MT49)
MSRVKLAVIGNGMVGHRFIEDLLEKADKDQFDITVFCEEPRIAYDRVHLSSYFSHHTAEE
LSLVREGFYEKQGVKVLVGERAITINRSEKVIHSNTGRTVYYDKLIMATGSYPWIPPIKG
SDSQDCFVYRTIEDLNAIEACARRSKRGAVVGGGLLGLEAAGALKSLGVETHVIEFAPVL
MAEQLDPMGGDQLRRKIERMGVKVHTGKNTQEIVNGGEQARKTMQFADGTALEVDFIVFS
TGIRAQDKLAHQCGLATARRGGIVINDSCQTSDPDVYAIGECASWRDRTFGLVAPGYKMA
QVTADHLLGRENTFLGADMSAKLKLLGVDVGGIGDAHGRTEGARSYVYLDESKEIYKRIV
VSADNKTLLGAVLVGDTSDYGNLLQLALNAIELPENPDGLILPSHAGSKPAIGVESLPEN
AQICSCFDVSKGDIIKAVSMGCHTVAAIKSETKAGTGCGGCIPLITQVLNAELTKQGIEV
NHHLCEHFAYSRQELYHLIRVEGIKSFDELLAKYGKGYGCEVCKPTVGSLLASCWNEYVL
KPQHTPLQDTNDNFLGNIQKDGTYSVIPRSAGGEITPDGLMAVGQIAKEYNLYTKMTGSQ
RIGMFGAQKDDLPAIWSKLIAAGFETGHAYAKALRMSKTCVGSTWCRYGVGDSVGFGVKL
EHRYKGIRTPHKMKFGVSGCTRECAEAQGKDVGIIATENGWNLYVCGNGGMKPRHADLLA
ADLDSETLEHYLDRFMMFYIRTADKLQRTSVWLESLEGGIDYLRKVIIDDKLGINAQLEA
EIARLRDAVICEWKETVEHPESQVRFAHFINSSQRDPNVQVVAEREQHRPARPDERIPVT
LLETEENNA