Protein Info for IAI46_22950 in Serratia liquefaciens MT49

Annotation: replicative DNA helicase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 474 TIGR00665: replicative DNA helicase" amino acids 29 to 467 (439 residues), 570.7 bits, see alignment E=9.7e-176 PF00772: DnaB" amino acids 30 to 131 (102 residues), 121.1 bits, see alignment E=2.9e-39 PF03796: DnaB_C" amino acids 209 to 465 (257 residues), 357.9 bits, see alignment E=4.3e-111 PF13481: AAA_25" amino acids 225 to 388 (164 residues), 38.8 bits, see alignment E=1.2e-13

Best Hits

Swiss-Prot: 88% identical to DNAB_SALTI: Replicative DNA helicase (dnaB) from Salmonella typhi

KEGG orthology group: K02314, replicative DNA helicase [EC: 3.6.4.12] (inferred from 98% identity to spe:Spro_4449)

MetaCyc: 86% identical to replicative DNA helicase (Escherichia coli K-12 substr. MG1655)
RXN0-4261 [EC: 5.6.2.3]

Predicted SEED Role

"Replicative DNA helicase (EC 3.6.1.-)" in subsystem DNA-replication (EC 3.6.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.4.12

Use Curated BLAST to search for 3.6.1.- or 3.6.4.12 or 5.6.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (474 amino acids)

>IAI46_22950 replicative DNA helicase (Serratia liquefaciens MT49)
MAGKKPTNKTNADEARERPRDRQMEGLKMPPHSLEAEQSVLGGLMLDNERWDNVAERVVS
NDFFSRPHRLIFTEMQRLLEMSRPIDLITLSESLEQRGELDSVGGFAYLAELSKNTPSAA
NIGAYADIVRERAVVREMISVANEIADAGYDPQGRSSEDLLDLAESRVFQIAENRASKDE
GPKGIERILEDTVARIEQLYQQPHDGVTGVDTGYQDLNKKTAGLQKSDLIIVAARPAMGK
TTFAMNLCEHAAMTQDKPVLIFSLEMPCNQIMMRMLASLSRVDQTRIRTGQLDDEDWARI
SSTMGILLEKRNMYIDDSSGLTPTEVRSRARRIFREHDGISLIMIDYLQLMRVPSLSDNR
TLEIAEISRSLKALAKELQVPVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMF
IYRDEVYHENSDLKGIAEIILGKQRNGPIGTVRLTFNGQWSRFDNYAGPQYDDE