Protein Info for IAI46_22795 in Serratia liquefaciens MT49

Annotation: glutamate--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00464: glutamate--tRNA ligase
  • amino acids 3 to 459 (457 residues), 584.1 bits, E=1.1e-179
• PF00749: tRNA-synt_1c
  • amino acids 3 to 304 (302 residues), 370.3 bits, E=6.6e-115
• PF19269: Anticodon_2
  • amino acids 325 to 460 (136 residues), 89.4 bits, E=3e-29

Best Hits

Swiss-Prot: 50% identical to SYE2_METCA: Glutamate--tRNA ligase 2 (gltX2) from Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)

KEGG orthology group: K01885, glutamyl-tRNA synthetase [EC: 6.1.1.17] (inferred from 50% identity to mca:MCA1234)

Predicted SEED Role

"Glutamyl-tRNA synthetase (EC 6.1.1.17)" in subsystem Heme and Siroheme Biosynthesis or tRNA aminoacylation, Glu and Gln (EC 6.1.1.17)

MetaCyc Pathways

• tRNA charging (21/21 steps found)
• superpathway of heme b biosynthesis from glutamate (10/10 steps found)
• tetrapyrrole biosynthesis I (from glutamate) (6/6 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
• adenosylcobalamin biosynthesis II (aerobic) (13/33 steps found)
• adenosylcobalamin biosynthesis I (anaerobic) (12/36 steps found)

KEGG Metabolic Maps

• Aminoacyl-tRNA biosynthesis
• Glutamate metabolism
• Porphyrin and chlorophyll metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.17

Use Curated BLAST to search for 6.1.1.17

Protein Sequence (466 amino acids)

>IAI46_22795 glutamate--tRNA ligase (Serratia liquefaciens MT49)
MSVRTRFAPSPTGFLHVGGARTALYSWLYARHNHGKFILRIEDTDVEREKEGAVDAILDS
MKWLGMYWDEGPFYQTKRLERYNEVVDQLLASGQAYRCYCSEERLNALKAQQEKNKENFI
YDRHCLHHLGHDPSLPHVVRLKSPQQGIVAWDDMIRGRIEFSNSEFDDFIIRRTDGWPTY
NFCVVVDDIDMQMTHIIRAEEHINNTPKQIYVYRALGLEPPVFAHVSPILGDDGKKLSKR
HGAANVMQYQEEGFLPEALRNYLVRLGWGHQDKEIFSLDEMISLFDINNVSNSPSAFNTE
KLLWLNKHYIAAATTEHLLPDLQWQLQRLAISTEDGPPLERVVMLLKTRARTMKEMAEAC
RSYYGEYQCNNQETYEQLLASAHPTVFCEIYQHFSTINDWQSAEINQVIGDISKEFGTSL
KDINMPLRLFILGAKDTPSLNDCLAVLGKEKTLNRLHAAIENMSCK