Protein Info for IAI46_22390 in Serratia liquefaciens MT49

Annotation: serine endoprotease DegQ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 456 signal peptide" amino acids 1 to 36 (36 residues), see Phobius details TIGR02037: peptidase Do" amino acids 40 to 454 (415 residues), 529.7 bits, see alignment E=2.9e-163 PF00089: Trypsin" amino acids 93 to 253 (161 residues), 73.9 bits, see alignment E=4.4e-24 PF13365: Trypsin_2" amino acids 93 to 229 (137 residues), 117.9 bits, see alignment E=1.6e-37 PF00595: PDZ" amino acids 264 to 345 (82 residues), 55.8 bits, see alignment E=1.2e-18 amino acids 378 to 444 (67 residues), 44.4 bits, see alignment E=4.4e-15 PF13180: PDZ_2" amino acids 287 to 359 (73 residues), 57.7 bits, see alignment E=2.9e-19 PF17820: PDZ_6" amino acids 294 to 333 (40 residues), 43.5 bits, see alignment 5.8e-15 amino acids 395 to 438 (44 residues), 33.4 bits, see alignment 8.1e-12

Best Hits

Swiss-Prot: 72% identical to DEGQ_ECOLI: Periplasmic pH-dependent serine endoprotease DegQ (degQ) from Escherichia coli (strain K12)

KEGG orthology group: K04772, serine protease DegQ [EC: 3.4.21.-] (inferred from 96% identity to spe:Spro_4352)

MetaCyc: 72% identical to periplasmic serine endoprotease (Escherichia coli K-12 substr. MG1655)
3.4.21.-

Predicted SEED Role

"Outer membrane stress sensor protease DegQ, serine protease" in subsystem Proteolysis in bacteria, ATP-dependent

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.-

Use Curated BLAST to search for 3.4.21.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (456 amino acids)

>IAI46_22390 serine endoprotease DegQ (Serratia liquefaciens MT49)
MKKKSLILSALAMSIGLTLTSVPAATAAMPVAVQGQPLPSLAPMLEKVLPAVVSVHVEGT
QVQRQQVPEEFRRFFGPGGPGQQQSSRPFEGLGSGVIIDAAKGYVLTNNHVINNADKISV
QLNDGREVDAKLVGRDEQSDIALLQLNDVKNLTAIKMADSDKLRVGDFAVAVGNPFGLGQ
TATSGIISALGRSGLNLEGLENFIQTDASINRGNSGGALVNLNGELIGINTAILAPSGGN
VGIGFAIPSNMAQNLSQQLIEFGEVKRGLLGIKGSEMTADMAKAFNTDAQRGAFVSEVLP
KSAAAKAGIKAGDILVSVDGKPVSSFAELRAKVGTTAPGKTIKVGLLRDGKPQEVSVTLD
NSESASTNAETLSPALQGVSLSNGAIPSGEKGVKIDKVDKGSAAAQIGLQKDDVIIGVNR
QRIENITALRKVLEAKPPVMALNIVRGSETIYLLLR