Protein Info for IAI46_22060 in Serratia liquefaciens MT49

Annotation: bifunctional [glutamate--ammonia ligase]-adenylyl-L-tyrosine phosphorylase/[glutamate--ammonia-ligase] adenylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 945 PF03710: GlnE" amino acids 34 to 277 (244 residues), 321.4 bits, see alignment E=4.1e-100 amino acids 554 to 806 (253 residues), 341.3 bits, see alignment E=3.4e-106 PF08335: GlnD_UR_UTase" amino acids 298 to 437 (140 residues), 114.6 bits, see alignment E=3.8e-37 amino acids 826 to 918 (93 residues), 32.3 bits, see alignment E=9.5e-12

Best Hits

Swiss-Prot: 78% identical to GLNE_PECAS: Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme (glnE) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K00982, glutamate-ammonia-ligase adenylyltransferase [EC: 2.7.7.42] (inferred from 78% identity to eca:ECA3585)

MetaCyc: 75% identical to fused glutamine synthetase deadenylase/glutamine synthetase adenylyltransferase (Escherichia coli K-12 substr. MG1655)
GSDEADENYLATION-RXN [EC: 2.7.7.89]; [Glutamate--ammonia-ligase] adenylyltransferase. [EC: 2.7.7.89, 2.7.7.42]

Predicted SEED Role

"Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42)" in subsystem Ammonia assimilation (EC 2.7.7.42)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.42 or 2.7.7.89

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (945 amino acids)

>IAI46_22060 bifunctional [glutamate--ammonia ligase]-adenylyl-L-tyrosine phosphorylase/[glutamate--ammonia-ligase] adenylyltransferase (Serratia liquefaciens MT49)
MLPLSSRLQVQAQSIVQRFQETQGADCVLSSQEQRVLASSDFVCDMLLSQPQWLETLRKQ
PPVADEWQHYAAWLQDTLEDVRDEAQLMRALRLFRRETLVRIAWAQAQNLCSTQETLLQL
SGLAETLIVSARDWLYQTCCREWGTPCNAQGVPQPLLILGMGKLGGGELNFSSDIDLIFA
YPENGQTQGGRRELDNAQFFTRLGQRLIKALDQQTIDGFVYRVDMRLRPFGDSGPLVMSF
AALEDYYQEQGRDWERYAMVKARLMGGSEDAYSEELRKTLRPFVFRRYIDFSVIQSLRNM
KGMIAREVRRRGLKDNIKLGAGGIREIEFITQVFQLIRGGREPALQGRSLLPTLQAVGEL
GLLEPQQVQAMSASYLFLRRLENLLQAIADQQTQTLPQDELDQARLAWGMGFDDWSTLLA
ALDEHMQAVRAVFNDLIGDDSPDVGEDPDYQHYHSLWQDALEEHELAPLTPHLDEEARRQ
MLRTIAEFRHDVDKRTIGPRGRDVLDQLMPRLLAEVCPRQDAPTALLRLTQLLLSIVTRT
TYLELLVEYHAALSHLIRLCAASPMVANQLSRYPLLLDELLDPATLYQPVAPDAYRSELR
QYLLRVPEDDEEQKLEALRQFKQAQQLRIAAGDISGALPVMKVSDHLTYLAEAIIDAVVQ
QAWNDMVARYGQPTHLQEREGRGFAVIGYGKLGGWELGYSSDLDLVFILDCPPDVMTDGD
RCIDGRQFYLRLAQRVMHLFSTRTSSGILYEVDARLRPSGAAGMLVSTVEAFADYQQNEA
WTWEHQALVRARIVHGDPALHQQFDAIRREILCKPRDGETLQLEVREMREKMRNHLGNKQ
RELFDIKADEGGITDIEFIAQYLVLGYASTEPRLTHWSDNVRIFELMANYGIMPEEEAQA
LTQAYVTMRDEIHHLALQEHSGKVAKGQFAAEREQVRTSWAKWLG