Protein Info for IAI46_21465 in Serratia liquefaciens MT49

Annotation: A/G-specific adenine glycosylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 370 TIGR01084: A/G-specific adenine glycosylase" amino acids 21 to 292 (272 residues), 418.1 bits, see alignment E=9.1e-130 PF00730: HhH-GPD" amino acids 52 to 184 (133 residues), 93.7 bits, see alignment E=1.3e-30 PF00633: HHH" amino acids 116 to 143 (28 residues), 30.6 bits, see alignment (E = 3.2e-11) PF14815: NUDIX_4" amino acids 249 to 359 (111 residues), 78.4 bits, see alignment E=5.4e-26

Best Hits

Swiss-Prot: 75% identical to MUTY_SALTY: Adenine DNA glycosylase (mutY) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K03575, A/G-specific adenine glycosylase [EC: 3.2.2.-] (inferred from 95% identity to spe:Spro_4043)

MetaCyc: 75% identical to adenine DNA glycosylase (Escherichia coli K-12 substr. MG1655)
RXN0-2661 [EC: 3.2.2.31]

Predicted SEED Role

"A/G-specific adenine glycosylase (EC 3.2.2.-)" in subsystem DNA repair, bacterial (EC 3.2.2.-)

Isozymes

Compare fitness of predicted isozymes for: 3.2.2.-

Use Curated BLAST to search for 3.2.2.- or 3.2.2.31

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (370 amino acids)

>IAI46_21465 A/G-specific adenine glycosylase (Serratia liquefaciens MT49)
MLQSRFIFCRISNQLMMQAQQFAQVVLDWYQRYGRKTLPWQLDKTAYQVWLSEVMLQQTQ
VATVIPYFQRFMARFPNVRALAEAPLDEVLHLWTGLGYYARARNLHKAAQAIVAQHGGEF
PTTFEEIHALPGIGRSTAGAVLSLALGQHYPILDGNVKRVLARCYAVEGWPGKKEVENRL
WEISENVTPAKGVGQFNQAMMDLGAMVCTRSKPKCELCPLSLGCIAYAHHSWAKYPGKKP
KQTLPEKTAYFLLLQHGERAWLEQRPAVGLWGGLFCFPQFSEREEMELWLQQRGLKNNRR
EQLTAFRHTFSHFHLDIVPIWLEMDAAGSSMDEGAGLWYNLAQPPSVGLAAPVDRLLQQL
AKQSPRQQGL