Protein Info for IAI46_21435 in Serratia liquefaciens MT49

Annotation: radical SAM family heme chaperone HemW

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 379 TIGR00539: putative oxygen-independent coproporphyrinogen III oxidase" amino acids 7 to 368 (362 residues), 470.7 bits, see alignment E=1.6e-145 PF04055: Radical_SAM" amino acids 10 to 180 (171 residues), 84.8 bits, see alignment E=8.2e-28 PF06969: HemN_C" amino acids 302 to 367 (66 residues), 39 bits, see alignment E=7.1e-14

Best Hits

Swiss-Prot: 83% identical to HEMW_ECOLI: Heme chaperone HemW (hemW) from Escherichia coli (strain K12)

KEGG orthology group: K02495, oxygen-independent coproporphyrinogen III oxidase [EC: 1.3.99.22] (inferred from 98% identity to spe:Spro_4035)

Predicted SEED Role

"Radical SAM family enzyme, similar to coproporphyrinogen III oxidase, oxygen-independent, clustered with nucleoside-triphosphatase RdgB" in subsystem Heat shock dnaK gene cluster extended or Heme and Siroheme Biosynthesis or Queuosine-Archaeosine Biosynthesis

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.22

Use Curated BLAST to search for 1.3.99.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (379 amino acids)

>IAI46_21435 radical SAM family heme chaperone HemW (Serratia liquefaciens MT49)
MLKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHQEYVDHLLADLDADLPLVSGREIS
TIFIGGGTPSLLSAEAMQSLLDGVRARIPVIDGAEITMEANPGTVEADRFSGYQRAGVNR
ISIGVQSFSAEKLTRLGRIHGPEEAKRAAHLATGLGLRSFNLDLMHGLPDQSLEEALDDL
RQAIALNPPHLSWYQLTIEPNTLFSSRPPVLPDDDALWDIFERGHELLSAAGYQQYETSA
YAKPGYQCQHNLNYWRFGDYLGIGCGAHGKLTFSDGRILRTAKTKHPRGFMQGKYMDKQH
EVTAADRPFEFFMNRFRLLEAAPRAEFVNYTGLDESAIRAALDEALAKGYLVETPEYWQI
TEKGKLFLNSLLELFLADE