Protein Info for IAI46_20485 in Serratia liquefaciens MT49

Annotation: bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 717 transmembrane" amino acids 39 to 61 (23 residues), see Phobius details amino acids 415 to 437 (23 residues), see Phobius details PF01553: Acyltransferase" amino acids 15 to 136 (122 residues), 70.8 bits, see alignment E=1e-23 PF00501: AMP-binding" amino acids 220 to 568 (349 residues), 193.9 bits, see alignment E=4e-61

Best Hits

Swiss-Prot: 80% identical to AAS_YERE8: Bifunctional protein Aas (aas) from Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)

KEGG orthology group: K05939, acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase / long-chain-fatty-acid--[acyl-carrier-protein] ligase [EC: 2.3.1.40 6.2.1.20] (inferred from 95% identity to spe:Spro_3830)

MetaCyc: 68% identical to fused 2-acylglycerophospho-ethanolamine acyltransferase/acyl-acyl carrier protein synthetase (Escherichia coli K-12 substr. MG1655)
RXN-5741 [EC: 6.2.1.47]; 6.2.1.- [EC: 6.2.1.47]; Acyl-[acyl-carrier-protein]--phospholipid O-acyltransferase. [EC: 6.2.1.47, 2.3.1.40]

Predicted SEED Role

"2-acylglycerophosphoethanolamine acyltransferase (EC 2.3.1.40) / Acyl-[acyl-carrier-protein] synthetase (EC 6.2.1.20)" (EC 2.3.1.40, EC 6.2.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.40 or 6.2.1.20 or 6.2.1.47

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (717 amino acids)

>IAI46_20485 bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase (Serratia liquefaciens MT49)
MIFSMLRVLFRLLYRVRVEGDARHFDQKKLLITPNHISFLDGILLALFLPIKPVFAVYAS
IGDRWFMRWLRPYIDFVSLDPTKPMAIKQLVRMVEQGRPIVVFPEGRITVTGSLMKIYDG
AAFIAAKSGATVVPIRLDGAEFSPFGRMAGVFKIRWFPQISIRILPPTSLPMPVAPRARE
RRMLAGDRLHQIMMKARMDTREPQTLFRALLAAQRRYGRSKPCIEDINFKEDSYQTLIKK
ALGVSRILQRFTAEGEHVGMLLPNATITAAAIFGASLRNRIPAMLNYTAGSNGLKSAMLA
TSLKTIVTSRQFLEKGKLTHLPQQVPEANWVYLEDLKDTVTLADKLWILRHLFQPARAML
PQQPDDAALILFTSGSEGNPKGVVHSHASLLANVEQIRTIADFTPRDRFMSSLPLFHSFG
LTVGLLTPLMTGSRIFLYPSPLHYRVVPELVYDRNCTVLFGTATFLNNYARFAHPYDFAR
LRYVVAGAEKLADSTKQIYQDKYGIRILEGYGVTECAPVVSINVPLAAKVGTVGRIMPNM
EARLMKVPGIENGGRLQLRGPNIMKGYLRVEHPGELEPPAAEDEQGNLQPGWYDTGDIVT
LDEQGYCVIRGRVKRFAKLAGEMVSLESVELLAQRVSPEGLHAATVKSDSSKGEALVLFT
TDASVTREALLWAARELGSPELAVPRDIRLLKSLPVLGSGKPDFVTLRKMAEQPETV