Protein Info for IAI46_20410 in Serratia liquefaciens MT49

Annotation: pitrilysin

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 962 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF00675: Peptidase_M16" amino acids 56 to 192 (137 residues), 117.8 bits, see alignment E=6.1e-38 PF05193: Peptidase_M16_C" amino acids 216 to 393 (178 residues), 88.3 bits, see alignment E=8.8e-29 amino acids 683 to 859 (177 residues), 53 bits, see alignment E=6.3e-18 PF16187: Peptidase_M16_M" amino acids 401 to 674 (274 residues), 205.4 bits, see alignment E=1.5e-64

Best Hits

Swiss-Prot: 68% identical to PTRA_ECOLI: Protease 3 (ptrA) from Escherichia coli (strain K12)

KEGG orthology group: K01407, protease III [EC: 3.4.24.55] (inferred from 72% identity to eca:ECA0994)

MetaCyc: 68% identical to protease 3 (Escherichia coli K-12 substr. MG1655)
Pitrilysin. [EC: 3.4.24.55]

Predicted SEED Role

"Protease III precursor (EC 3.4.24.55)" (EC 3.4.24.55)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.24.55

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (962 amino acids)

>IAI46_20410 pitrilysin (Serratia liquefaciens MT49)
MRRQLARITGLVLLVMFWAPLSWAAQGWQPLAEKINKSDHDPRQYQAIKLTNGMTVLLVS
DAQAPKSLAALALPVGSLEDPNSQLGLAHYLEHMVLMGSKRYPQPENLSEFLKKHGGSHN
ASTASYRTAFYLEVENDALAPAVDRMADAIAEPILDPGNADRERNAVNAELTMARSRDGM
RMAQVGAETLNPAHPSARFSGGNLDTLKDKPGSKLHDELTTFYQRYYSANLMMGVLYSNQ
PLPALAELAAKTFGRVPNHDASVPPITVPAVTPEQQGIIIHYVPAQPRKQLKVEFRIDNN
SAEFRSKTDTYIAYLIGNRSQNTLSDWLQKQGLADAINAGADPMVDRNGGVFSISVSLTD
KGLAKRDEVVAAIFNYLKMLRSEGIKQSYFDEISHVLNLDFRYPSITRDMDYIEWLVDTM
LRVPVEHTLDAPYLADRYDAKAIAERLDAMTPQNARIWFVSPNEPHNKTAYFVNAPYQVD
KITPQRFEQWQQLGKGINLSLPALNPYIPDDFSLTKPSHEFKKPEMVVDQPGLRVLYMPS
RYFADEPKADVTVAFRNAKTMDSARNQVLFSLTDYLAGISLDQLSYQASVGGLSFSTSPN
NGLQFNANGFTQRLPQLLTSLIEGYSSFTPTEDQLAQAKSWYLEQLDSAEKGKAFELAIQ
PVQMVSRVPYSERSERREVLKTLTLKDVLAYRDSLLADATPELLVVGNMSKQQVDTLAST
LKHRLGCTGIEWWHGEDVEVAKKQLANLQRAGSSTDSALAAVYVPTGYDEVTGMAYSSLL
GQIIQPWFYSQLRTQEQLGYAVFAFPMSVGRQWGIGFLLQSNSKQPAYLYQRYQDFYPKT
EKRLREMKPADFEQYKQAMINELKQRPQTLSEEAGRFANDFDRGNFAFDTRHKLIEQIKQ
LTPVKLADYFHQAVIQPQGLAVLSQVSGSGQEKADYAVQPEWVTYPNASALQQTMPRKVA
TP