Protein Info for IAI46_20405 in Serratia liquefaciens MT49

Annotation: exodeoxyribonuclease V subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1183 TIGR00609: exodeoxyribonuclease V, beta subunit" amino acids 9 to 1172 (1164 residues), 1352.3 bits, see alignment E=0 PF00580: UvrD-helicase" amino acids 16 to 436 (421 residues), 168.8 bits, see alignment E=4.2e-53 PF13245: AAA_19" amino acids 21 to 423 (403 residues), 43.4 bits, see alignment E=7.4e-15 PF13361: UvrD_C" amino acids 441 to 626 (186 residues), 29.3 bits, see alignment E=1.2e-10 amino acids 681 to 808 (128 residues), 45.4 bits, see alignment E=1.5e-15

Best Hits

Swiss-Prot: 64% identical to RECB_ECOLI: RecBCD enzyme subunit RecB (recB) from Escherichia coli (strain K12)

KEGG orthology group: K03582, exodeoxyribonuclease V beta subunit [EC: 3.1.11.5] (inferred from 69% identity to eca:ECA0995)

MetaCyc: 64% identical to exodeoxyribonuclease V subunit RecB (Escherichia coli K-12 substr. MG1655)
RXN-11135 [EC: 5.6.2.4]; Exodeoxyribonuclease V. [EC: 5.6.2.4, 3.1.11.5]; RXN-19004 [EC: 5.6.2.4, 3.1.11.5]

Predicted SEED Role

"Exodeoxyribonuclease V beta chain (EC 3.1.11.5)" in subsystem DNA-replication (EC 3.1.11.5)

Isozymes

Compare fitness of predicted isozymes for: 3.1.11.5

Use Curated BLAST to search for 3.1.11.5 or 5.6.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1183 amino acids)

>IAI46_20405 exodeoxyribonuclease V subunit beta (Serratia liquefaciens MT49)
MTETTPQRLDPLTLPLFGERLIEASAGTGKTFTIGALYLRLLLGLGQAAAFPRPLTVEEI
LVVTFTEAATEELRGRIRSNIHGLRIACVRGRSKDPLFTSLMAEIKDLPDAAAQLLAAER
QMDEAAIYTIHGFCQRMLTHNAFESGILFEQTLVQDELPLRRQACADFWRRHCYPLPLGV
ARAVSQEWSGPEALLADLSGYLHGEAPLLRRPPKDDETVLMRHEQIVARIDAIKAQWVSA
AGDLEALISQSGVDKRSYSSKHLPNWLHKVGEWTVLETQDYQLPKELEKFRQSVLLDKTK
KGEPPRHPLFVAIDELFDEPLTLRDLIMARALSEIRLSIQQEKRQRAELGFDDLLSRLDS
ALHSEGGDRLAQAIRQRYPVAMIDEFQDTDPQQYRIFQKLYVGRPECGLLLIGDPKQAIY
AFRGADIFTYMRARSEVSAHYTLETNWRSSPAMVGSVNHLFAQVEKPFLFSQIPFIDVAA
AAKNRGLAFELQNQPQPAMQFWLQQGEGVGVSEYQQLMARLCATQIRDWLSAGQQQQAWL
VDGDKRRAVQASDITVLVRSRNEAALVRDALSALAIPSVYLSNRDSVFDTPEAKDLLWLL
QAVLAPEQERTLRSAMATGLMGLDALVLDSLNQDERAWDRLVDEFDGYRSLWLRRGVLPM
LREVMKQRHLAENLLATQGGERRLTDVMHLGELLQEASAQLDSEHALVRWLAQQIAQPNR
QSDNQQLRLESDRHLVQVITIHKSKGLEFDLVWLPFVGNFRQQQQVLYHDRQSFQALLDL
NADEESIEWAEEERLAEDLRLLYVALTRSVYHCSIGIAPLIQGTRKKQGDTDLHRSALGY
LVQAGQAGDAAYLQQCLQQLIREGVALSLVDTLDEQPWLPQTPELSELAAKGFSRQVQDF
WRVTSYTGLQQHGAGLMQDLLPRLDVDAAGEQAQDSEPALTPHTFPRGATPGTFLHSLFE
TLDFTQPLDEQWLLEQLQQQGFAEHWQPILLAWMQVLLNTPLNDAGVVLSALAPQQKQAE
LQFYLPIDRLLQARELDALVKNYDPLSARCPALDFQQVQGMLKGFIDLVFCWQGKYYLLD
YKSNWLGEDSSAYTRPAMEQAMAEHRYDLQYQLYTLALHRYLRHRLVDYDYQRHFGGVIY
LFLRGVDAQHPGNGIFACRPEQALVEGMDRLFSGEAITAEDEP