Protein Info for IAI46_20400 in Serratia liquefaciens MT49

Annotation: exodeoxyribonuclease V subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 616 PF21185: RecD_N" amino acids 5 to 112 (108 residues), 125.2 bits, see alignment E=6e-40 TIGR01447: exodeoxyribonuclease V, alpha subunit" amino acids 5 to 612 (608 residues), 642.6 bits, see alignment E=3.4e-197 PF13245: AAA_19" amino acids 159 to 309 (151 residues), 122 bits, see alignment E=9.8e-39 PF13604: AAA_30" amino acids 161 to 321 (161 residues), 99.4 bits, see alignment E=1.2e-31 PF00580: UvrD-helicase" amino acids 162 to 248 (87 residues), 27.7 bits, see alignment E=1.1e-09 PF13401: AAA_22" amino acids 168 to 302 (135 residues), 30.1 bits, see alignment E=2.6e-10 PF09848: SLFN-g3_helicase" amino acids 170 to 277 (108 residues), 20.4 bits, see alignment E=1.5e-07 PF05127: Helicase_RecD" amino acids 173 to 290 (118 residues), 42.6 bits, see alignment E=3.1e-14 PF01443: Viral_helicase1" amino acids 540 to 588 (49 residues), 31 bits, see alignment 1.1e-10 PF13538: UvrD_C_2" amino acids 541 to 587 (47 residues), 53.4 bits, see alignment 9.1e-18

Best Hits

Swiss-Prot: 63% identical to RECD_ECOLI: RecBCD enzyme subunit RecD (recD) from Escherichia coli (strain K12)

KEGG orthology group: K03581, exodeoxyribonuclease V alpha subunit [EC: 3.1.11.5] (inferred from 92% identity to srs:SerAS12_4003)

MetaCyc: 63% identical to exodeoxyribonuclease V subunit RecD (Escherichia coli K-12 substr. MG1655)
RXN0-4261 [EC: 5.6.2.3]; Exodeoxyribonuclease V. [EC: 5.6.2.3, 3.1.11.5]; RXN-19004 [EC: 5.6.2.3, 3.1.11.5]

Predicted SEED Role

"Exodeoxyribonuclease V alpha chain (EC 3.1.11.5)" in subsystem DNA-replication (EC 3.1.11.5)

Isozymes

Compare fitness of predicted isozymes for: 3.1.11.5

Use Curated BLAST to search for 3.1.11.5 or 5.6.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (616 amino acids)

>IAI46_20400 exodeoxyribonuclease V subunit alpha (Serratia liquefaciens MT49)
MIALLEQAQALGVLRPLDLQFARVVATADQPDILLAAACLSAEAGAGHVCLMLEQLLPEN
LFDGRQPELAQAVWLAAGQPDLTDWQQRLSASAAVGDGSTAMPMVLQQQRLYLQRMWQNE
GEVADFISGDSLHQAVEEAELRTILDQLFGEATDEPDWQKIAAAVAATRRIAVISGGPGT
GKTTTVAKLLAALVQLAEGRRLRIQLAAPTGKAAARLTESLGSAGRQLALTPEQRALFPT
EASTLHRLLGAQPNSQRMRYHRGNPLHLDVLVVDEASMVDLPMMARLIAALPAQARVIFL
GDRDQLASVEAGAVLGDICRFAEQGYSVARAEQLSRLTGCLLQGQSAEAEAAVRDSLCLL
RKSYRFDANSGIGQLAFAVNAGDGKKAAWALGGNFSDVTGHPLAETQDYQALLDACVAGY
RDYLQQVATGADAVTILAAFGQFQVLCALREGPFGIAGLNERIETGLQRAGLIQRKPGTA
GRWYPGRPVMIGRNDSALGLFNGDIGIALRDESGELRVHFQLPDGSIKSVQPSRLPAHET
AYAMTVHKSQGSEFDHTVLVLPNHFLPVLTRELVYTAITRARKQLSLYATEAVLLRAIRT
PTQRRSGLAERLQITG