Protein Info for IAI46_20290 in Serratia liquefaciens MT49

Annotation: tRNA pseudouridine(65) synthase TruC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00849: PseudoU_synth_2
  • amino acids 10 to 168 (159 residues), 118.2 bits, E=2e-38

Best Hits

Swiss-Prot: 79% identical to TRUC_YERPE: tRNA pseudouridine synthase C (truC) from Yersinia pestis

KEGG orthology group: K06175, tRNA pseudouridine synthase C [EC: 5.4.99.12] (inferred from 92% identity to spe:Spro_3796)

MetaCyc: 74% identical to tRNA pseudouridine65 synthase (Escherichia coli K-12 substr. MG1655)
RXN-11840 [EC: 5.4.99.26]

Predicted SEED Role

"tRNA pseudouridine synthase C (EC 4.2.1.70)" (EC 4.2.1.70)

MetaCyc Pathways

• pseudouridine degradation (2/2 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12 or 5.4.99.26

Protein Sequence (256 amino acids)

>IAI46_20290 tRNA pseudouridine(65) synthase TruC (Serratia liquefaciens MT49)
MIEVLYQDEHLVAVNKPSGWLVHRSWLDRHETRFVMQTVRDQIGQHVFTVHRLDRPTSGV
LLMALSSEVARLLSQQLEQHQMQKTYHAVVRGYVLEGDTLDYPLTEELDKIADKFASADK
GPQPAVTHYRPLAQAEMPVAIGRYDSARYSLVELKPEHGRKHQLRRHMVHIRHPIIGDSK
HGDLRQNRGMAEHFGCSRLMLHASHLQLNHPVTGEPLQISARWDESWQGVMSQFGWAESI
PELARVEFPSASGQDN