Protein Info for IAI46_20185 in Serratia liquefaciens MT49

Annotation: DNA polymerase III subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1162 TIGR00594: DNA polymerase III, alpha subunit" amino acids 6 to 1033 (1028 residues), 1414.6 bits, see alignment E=0 PF02811: PHP" amino acids 8 to 172 (165 residues), 154.1 bits, see alignment E=1.3e-48 PF07733: DNA_pol3_alpha" amino acids 295 to 558 (264 residues), 346.2 bits, see alignment E=4.2e-107 PF17657: DNA_pol3_finger" amino acids 561 to 735 (175 residues), 220.7 bits, see alignment E=2.2e-69 PF14579: HHH_6" amino acids 808 to 900 (93 residues), 90.7 bits, see alignment E=1.7e-29 PF01336: tRNA_anti-codon" amino acids 1003 to 1074 (72 residues), 38.3 bits, see alignment 3.2e-13 PF20914: DNA_pol_IIIA_C" amino acids 1102 to 1153 (52 residues), 102.7 bits, see alignment (E = 2.3e-33)

Best Hits

Swiss-Prot: 88% identical to DPO3A_SALTY: DNA polymerase III subunit alpha (dnaE) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K02337, DNA polymerase III subunit alpha [EC: 2.7.7.7] (inferred from 77% identity to avr:B565_3040)

MetaCyc: 88% identical to DNA polymerase III subunit alpha (Escherichia coli K-12 substr. MG1655)
DNA-directed DNA polymerase. [EC: 2.7.7.7]

Predicted SEED Role

"DNA polymerase III alpha subunit (EC 2.7.7.7)" in subsystem DNA-replication (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1162 amino acids)

>IAI46_20185 DNA polymerase III subunit alpha (Serratia liquefaciens MT49)
MAEPRFIHLRVHSDYSMIDGLAKIGPLVKRAAALAMPALAITDFTNLCGLVKFYGSAHGA
GIKPIIGADFHVQSEVLGDELAQLTVLAANNEGYQNLTLLISRAYQRGYGAAGPTIDRDW
LIEHREGLILLSGARQGDVGKFLLRGNQPQVEQCLEFYQQYFPDSYYLELIRTGRPDEEN
YLHAAVALATERGLPVVATNDVRFLVEDDFDAHEIRVAIHDGFTLDDPKRPRNYTAQQYM
RNEDEMCELFADIPEALLNSVEIAKRCNVTIRLGEYFLPQFPTGDMSTEDFLILKSKEGL
EDRLEFLFPDPEVRAQRRPEYDERLDIELNVINQMGFPGYFLIVMEFIQWSKDNNVPVGP
GRGSGAGSLVAYALKITDLDPLEFDLLFERFLNPERVSMPDFDVDFCMEKRDLVIEHVAD
MYGREAVSQIITFGTMAAKAVIRDVGRVLGHPYGFVDRISKLVPPDPGMTLEKAFAAEPQ
LPEIYEADEEVKALIDMARKLEGVTRNAGKHAGGVVIAPTKITDFAPLYCDAEGQHPVTQ
FDKNDVEYAGLVKFDFLGLRTLTIIDWALEMINARRAKTGLEPIDIAAIPLEDKKSFDML
QRSETTAVFQLESRGMKDLIKRLKPDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREE
ISYPDIQWQHESLKPVLEPTYGIILYQEQVMQIAQVLAGYSLGGADMLRRAMGKKNPVEM
AKQRGGFEDGAKARGIDGELSVKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPA
EFMAAVMTADMDNTDKVVGLVDECWRMGLKILPPDINSGQYHFHVNDEGEIVYGIGAIKG
VGEGPIEAILEARNSGEQGYFKDLFDLCARSDIKKINRRILEKLIMSGAFDRLGPHRAAL
MNSLPDALKAADQHAKAEAIGQVDMFGVLAEAPEQVEQSYANVPRWQEQVVLDGERETLG
LYLTGHPITQYLKEIERYAGGQRLKDMHPTDRGKMTTAVGLVIASRVMVTKRGNRIGICT
LDDRSGRLEVMLFTDALEKYQHLLEKDRILIASGQVSFDDFSGGLKMTARELMDISEARE
KYARGLAISLTDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQREDARAKLRFGATWRVTP
TDRLLIDLRTLVGNEQVELEFD