Protein Info for IAI46_19475 in Serratia liquefaciens MT49

Annotation: phosphoribosylformylglycinamidine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1297 TIGR01735: phosphoribosylformylglycinamidine synthase" amino acids 4 to 1295 (1292 residues), 2074 bits, see alignment E=0 PF18076: FGAR-AT_N" amino acids 36 to 150 (115 residues), 155 bits, see alignment E=1.5e-49 PF18072: FGAR-AT_linker" amino acids 171 to 220 (50 residues), 82.4 bits, see alignment (E = 5.2e-27) PF02769: AIRS_C" amino acids 432 to 588 (157 residues), 115.2 bits, see alignment E=6.7e-37 amino acids 835 to 968 (134 residues), 57.2 bits, see alignment E=4.5e-19 PF13507: GATase_5" amino acids 1041 to 1294 (254 residues), 367 bits, see alignment E=8.9e-114

Best Hits

Swiss-Prot: 84% identical to PUR4_ECO57: Phosphoribosylformylglycinamidine synthase (purL) from Escherichia coli O157:H7

KEGG orthology group: K01952, phosphoribosylformylglycinamidine synthase [EC: 6.3.5.3] (inferred from 73% identity to aap:NT05HA_1480)

MetaCyc: 84% identical to phosphoribosylformylglycinamide synthetase (Escherichia coli K-12 substr. MG1655)
Phosphoribosylformylglycinamidine synthase. [EC: 6.3.5.3]

Predicted SEED Role

"Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3) / Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3)" in subsystem De Novo Purine Biosynthesis (EC 6.3.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.5.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1297 amino acids)

>IAI46_19475 phosphoribosylformylglycinamidine synthase (Serratia liquefaciens MT49)
MMEILRGSPALSAFRVTKLLSRCQDAQLPVSDIYAEYVHFADVSAPLNAEEHGKLTRLLK
YGPSLAEHAPEGRLLLVTPRPGTISPWSSKATDIAHNCGLKQVVRLERGLAFYVKAPTLT
EAQWQQLAALLHDRMMETVFSSLQQAEQLFAQHQPAPYQAIDMLGEGRSALEKANLKLGL
ALAEDEIDYLLNAFTGLGRNPTDIELYMFAQANSEHCRHKIFNADWIIDGEQQPKSLFKM
IKNTFEQTPDYVLSAYKDNAAVMEGSQVGRFFAAPHSGQYDYHQEEAHILMKVETHNHPT
AISPWPGAATGSGGEIRDEGATGRGAKPKAGLVGFSVSNLRIPGFEQPWEQDFGKPDRIV
TALDIMTDGPLGGAAFNNEFGRPALVGYFRTYEEQVNSHNGVELRGYHKPIMLAGGIGNI
RADHVQKGEITVGAKLVVLGGPAMNIGLGGGAASSMASGQSDADLDFASVQRDNPEMERR
CQEVIDRCWQLGEQNPILFIHDVGAGGLSNAMPELVSDGGRGGRFELRDILNDEPGMSPL
EVWCNESQERYVMAIAPAQMAQFDEICRRERAPYAVIGEATEEQHLTLNDSHFDNQPIDM
PLDVLLGKTPKMTRDVTRLQAKGQPVQRDNITLADAVKRVLHLPAVAEKTFLITIGDRTV
TGMVARDQMVGPWQIPVADCAVTTASLDSYYGEAMSIGERAPVALLDFAASGRLAVGEAL
TNLAATEIGSLKRVKLSANWMAAAGHPGEDAGLYEAVKAVGEELCPALGITIPVGKDSMS
MKTRWQEGNEQREMTSPLSLVITGFARVEDVRHTVTPQLRTDKGDTALLLVDLGNGHNAL
GATALAQVYRQLGDKPADVRNVEQLAGFFNAMQQLVADRALLAYHDRSDGGLLVTLAEMA
FAGHCGVQVDIQGLGDDVLAALFNEELGAVIQVTADRLEAVQQAFAQHGLTDNVHHIGSV
QAGDRFVITQGDKPVYSESRATLRTWWAETTWQMQRLRDNPECADQEHQAKQNERDPGLN
VKLSFAPEEDIAAPYIAKGARPKVAVLREQGVNSHVEMAAAFHRAGFDAVDVHMSDLLEG
RRDLQDFHTLVACGGFSYGDVLGAGEGWAKSILFNERVRDEFESFFHRPQTLALGVCNGC
QMMSNLRDLIPGAEHWPRFVRNLSDRFEARFSLVEVAASPSLFMQGMAGSRMPIAVSHGE
GHVEVRDTAHLAALEHHGLVALRFVDNAGQVTESYPANPNGSPNGITAVTSANGRVTVMM
PHPERVFRTVSNSWHPEEWGEDSPWMRMFRNARKQLG