Protein Info for IAI46_19355 in Serratia liquefaciens MT49
Annotation: IscS subfamily cysteine desulfurase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 99% identical to ISCS_SERP5: Cysteine desulfurase IscS (iscS) from Serratia proteamaculans (strain 568)
KEGG orthology group: None (inferred from 99% identity to srr:SerAS9_3831)MetaCyc: 94% identical to cysteine desulfurase IscS (Escherichia coli K-12 substr. MG1655)
Cysteine desulfurase. [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]
Predicted SEED Role
"Cysteine desulfurase (EC 2.8.1.7), IscS subfamily" in subsystem Alanine biosynthesis or Thiamin biosynthesis (EC 2.8.1.7)
MetaCyc Pathways
- superpathway of thiamine diphosphate biosynthesis I (10/10 steps found)
- superpathway of thiamine diphosphate biosynthesis II (10/11 steps found)
- thiazole component of thiamine diphosphate biosynthesis I (6/6 steps found)
- superpathway of L-alanine biosynthesis (4/4 steps found)
- thiazole component of thiamine diphosphate biosynthesis II (6/7 steps found)
- molybdopterin biosynthesis (5/6 steps found)
- L-alanine biosynthesis III (1/1 steps found)
- L-cysteine degradation IV (1/1 steps found)
- cytidylyl molybdenum cofactor sulfurylation (1/2 steps found)
- tRNA-uridine 2-thiolation and selenation (bacteria) (7/11 steps found)
- bis(guanylyl molybdopterin) cofactor sulfurylation (1/3 steps found)
- tRNA-uridine 2-thiolation (mammalian mitochondria) (1/4 steps found)
- tRNA-uridine 2-thiolation (yeast mitochondria) (1/4 steps found)
- tRNA-uridine 2-thiolation (thermophilic bacteria) (1/5 steps found)
- [2Fe-2S] iron-sulfur cluster biosynthesis (4/10 steps found)
- tRNA-uridine 2-thiolation (cytoplasmic) (1/8 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.8.1.7
Use Curated BLAST to search for 2.8.1.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (404 amino acids)
>IAI46_19355 IscS subfamily cysteine desulfurase (Serratia liquefaciens MT49) MKLPIYLDYSATTPVDPRVAEKMMQFLTLDGTFGNPASRSHRFGWQAEEAVDIARNQIAE LVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKTEHKAVLDTCRQLEREGFE VTYLAPQSNGIISLQALEAAMRDDTILVSIMHVNNEIGVVQDIEAIGEMCRARGIIYHVD ATQSVGKLPIDLSKLKVDLMSFSGHKIYGPKGIGALYVRRKPRIRIEAQIHGGGHERGMR SGTLPVHQIAGMGEAYRIAKEEMPTEMARLRTLRDRLWNGVKDMEEVYLNGDLEHGVPNI LNVSFNYVEGESLIMALKDLAVSSGSACTSASLEPSYVLRALGMTDELAHSSIRFSLGRF TTEEEIDYTIQLVRKSIGRLRDLSPLWDMFKQGVDINSIEWAHH